美托洛尔对异丙基肾上腺素充血性心力衰竭大鼠模型TNF-α的影响  

Effect of metoprolol on plasma TNF-α in rat model with isoproterenol-induced congestive heart failure

作　　者：蔡辉[1] 郭寒[1] 赵智明[1] 蔡佳宇[1] 郭郡浩[1] 

机构地区：[1]南京军区南京总医院中西医结合科,江苏南京210002

出　　处：《徐州医学院学报》2010年第1期34-37,共4页Acta Academiae Medicinae Xuzhou

基　　金：南京军区南京总医院科研基金(2008042)

摘　　要：目的探讨美托洛尔对充血性心力衰竭(心衰)大鼠血浆肿瘤坏死因子-α(TNF-α)的影响。方法雄性SD大鼠80只,随机分为空白组(n=10)及模型组(n=70),模型组皮下注射异丙基肾上腺素2次(170 mg.kg-1),6周后应用超声心动图检测,以左室射血分数≤45%确定造模成功,将心衰大鼠模型随机分为心衰对照组(n=13)、美托洛尔组(n=14);18周后使用左心导管进行血流动力学检查,利用放免法检测血浆TNF-α的改变并观察心肌病理形态学特征。结果①与空白组比较,心衰对照组心功能明显恶化,心室质量指数、左心室质量指数及血浆TNF-α显著升高(P<0.01),心室重构明显;②与心衰对照组比较,美托洛尔组心功能明显好转,心室质量指数、左心室质量指数及血浆TNF-α明显降低(P<0.05),心室重构好转。结论美托洛尔能改善心功能,逆转心室重构,其作用可能与抑制心衰大鼠血浆TNF-α表达有关。Objective To explore the effect of metoprolol on plasma tumour necrosis factor-α（TNF-α） in rats with congestive heart failure（CHF）.Methods 80 male SD rats were randomly allocated to the isoproterenol（ISO）-induced heart failure group（n=70） receiving a subcutaneous injection of ISO（170 mg per kilogram of body weight） twice and the healthy normal group（n=10） receiving a subcutaneous injection of 0.25 ml of normal saline twice.At the 6th week,the left ventricular ejection fractions（LVEF） were measured by echocardiogram,the remaining rats,with a LVEF≤45%,were randomly divided into two groups： heart failure control group（n=13）,metoprolol group（n=14,8 mg/kg-1· d-1,i.g.,）.At the 18th week,the hemodynamic parameters were detected,including HR,LVEDP,LVSP and ±dp/dtmax;LVWI was measured after LV morphopathological examination.TNF-α in plasma was examined by radioimmunoassay.Results ①Compared with the normal group,the heart function significantly deteriorated;VWI,LVWI and the plasma TNF-α significantly increased（P〈0.01） in the heart failure control group.②Compared with the heart failure control group,the heart function markedly improved,and the VWI,LVWI and the plasma TNF-α decreased significantly（P〈0.05） in the metoprolol group,with an improved ventricular remodeling.Conclusion Metoprolol can inhibit the plasma TNF-α,improve heart function and reverse ventricular remodeling with CHF.Its effect might be involved in the inhibition of the expression of plasma TNF-α in hear failure rats.

关 键 词：充血性心力衰竭 异丙基肾上腺素 美托洛尔 大鼠 肿瘤坏死因子-Α 心室重构 

分 类 号：R331.31[医药卫生—人体生理学]