bFGF-PLGA缓释微球及其体外释药性能研究  

Preparation of bFGF-PLGA controlled-release microspheres and their sustained release performance in vitro

作　　者：武继民[1,2] 汪鹏飞[2] 李志宏[2] 袁晓燕[1] 

机构地区：[1]天津大学材料科学与工程学院,天津300072 [2]军事医学科学院卫生装备研究所,天津300161

出　　处：《中国生化药物杂志》2010年第1期32-34,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金(30970724);天津市自然基金(08JCYBJC3400)项目资助

摘　　要：目的以bFGF为缓释药物、PLGA为药物载体制备bFGF-PLGA缓释微球,观察微球表面形态,检测微球物理性能和体外释药行为。方法采用W1/O/W2复乳溶剂挥发法制作微球;通过扫描电镜观察微球的表面形态结构;利用ELISA法测试微球中药物的载药量和包封率,并对微球中药物的体外释放行为进行研究。结果微球表面圆滑均匀,平均粒径(0.75±0.08)μm,载药量[(59.9±1.9)×10-3]%,包封率为(79.9±2.8)%;在为期45 d的体外释放试验中,bFGF累积释放率达到80%。结论bFGF-PLGA微球能够稳定地在较长时间释放药物bFGF,验证了PL-GA微球作为bFGF控制释放载体的可行性。Purpose To prepare bFGF-PLGA microspheres and to investigate the characteristics. Methods The bFGF-PLGA microspheres were prepared by W1/O/W2 multiple emulsion volatilizing method, the morphology was investigated using scanning electron microscope （SEM）, the ELISA method was used to establish the regression equation and to detect the drug loading amount and encapsulation efficiency, as well as sustained-release profile in vitro. Results The microspheres seemed to be smooth and uniform with mean particle size of （0.75±0.08）μm, the the drug loading amount and encapsulation efficiency were [ （59.9 ± 1.9） × 10-3] % and （79.9 ±2.8） % ,respectively ,tl,e accumulative release ratio was up to 80% in the continuous period of forty-five days. Conclusion The bFGF-PLGA microspheres have better pharmaceutical properties and long-time sustained release effect in vitro.

关 键 词：BFGF PLGA微球 缓释 

分 类 号：R944.9[医药卫生—药剂学]