HPLC法测定田参正脉含片中二氢杨梅素的含量  

Determination of dihydromyricetine in renshen buccal tablet by HPLC

作　　者：冯炜业[1] 王立兴 

机构地区：[1]福建医科大学附属第一医院药学部,福州350004 [2]福州长富星新药开发有限公司,福州350002

出　　处：《海峡药学》2010年第1期47-49,共3页Strait Pharmaceutical Journal

摘　　要：目的建立人参含片中二氢杨梅素的含量测定方法。方法采用高效液相色谱法，色谱柱为Lichrospher C18柱（4．6×250mm，5μm）。ODS预柱，流动相为甲醇0．1％磷酸（25：75）；检测波长290nm；流速为1．0mL·min^-1。结果本法精密度高，稳定性好。二氢杨梅素溶液在0．00532～0．1064mg·mL^-1的浓度范围内具有良好的线性关系（r=0．9997），平均加样回收率为101．62％，RSD为1．55％（n=6）。结论本方法简便，准确，重现性好，可作为该制剂的含量测定方法。OBJECTIVE To establish a method for determination of Dihydromyricetine in Renshen buccal tablet,METHODS Using a HPLC method with Lichrospher C18（4.6 × 250mm, 5μm）column, ODS pre-column; the mobile phase was methanol -0.1 phosphoric acid （25：75）;the detection wavelength was set at 290nm; flow rate was 1.0mL· min^-1. The precision and stability were fine.the linean range within 0.00532- 0. 1064mg· mL^-1,r = 0.9997.The average rocoveries of dihydromyricetine（n = 6） was 101.62% with RSD 1.55% .CONCLUSION The method was simple, accurate and reproducible, and can be used to determine the dihydromyricetine in Tianshen Zhengmai buccal tablet.

关 键 词：田参正脉含片 二氢杨梅素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]