重组鸡白细胞介素-2对禽流感灭活疫苗免疫增强作用的研究  

Immunopotentiation of Recombinant Chicken Interleukin-2 on Avian Influenza Inactivated Vaccine

作　　者：牛泽[1] 任晓峰[1] 曾岩[1] 刘明 卢春颖 李广兴[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]哈尔滨兽医研究所国家禽流感参考实验室,黑龙江哈尔滨150001 [3]内蒙古锡林郭勒盟第二中学,内蒙古锡林浩特026000

出　　处：《中国家禽》2010年第3期20-23,共4页China Poultry

基　　金：黑龙江省自然科学基金重点资助项目(ZNJ0702-01)

摘　　要：为研究重组鸡白细胞介素-2(IL-2)对H5N1亚型禽流感灭活苗的免疫效果,将重组IL-2蛋白和禽流感灭活疫苗联合免疫14日龄SPF鸡,免疫后每周测定一次血清抗体效价﹑脾脏T淋巴细胞亚群,最后计算攻毒保护率。试验结果表明:重组IL-2蛋白具有增强禽流感疫苗免疫效果的作用,提高了血清中的抗体水平﹑增加了脾脏T淋巴细胞CD4+﹑CD8+亚群数量。The purpose of the study was to evaluate immune effect of recombinant chicken interleukin-2（IL-2） on an inactivated vaccine against HSN1 subtype avian influenza. The recombinant IL-2 protein and avian influenza inactivated vaccine were used as immunogens to immunize 14-day-age specific pathogen free （SPF） chickens. The titers of serum antibody against avian influenza virus and the number of spleen T-lymphocyte subsets were measured at one-week interval. In addition,the protection rate of the vaccine to the chickens post-chaflenge was calculated. The results indicated that recombinant IL-2 enhanced the serum antibody titers significantly and increased the spleenic T-lymphocyte CD4^＋ and CD8^＋cells of immunized chickens.

关 键 词：鸡白细胞介素-2 禽流感灭活疫苗 免疫效果 

分 类 号：S859.797[农业科学—临床兽医学] S852.4[农业科学—兽医学]