山梨SRAP反应体系的建立  

Establishment and Optimization of SRAP Reaction System in Pyrus ussuriensis

作　　者：徐颖[1] 韩影[1] 赵巍巍[1] 宗成文[1] 曹后男[1] 

机构地区：[1]延边大学农学院,吉林龙井133400

出　　处：《湖北农业科学》2010年第1期24-26,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30560091)

摘　　要：以山梨为试材,提取其基因组DNA,探讨了SRAP反应体系中各组分浓度对扩增的影响。结果表明,采用该方法提取的基因组DNA纯度好、片段完整、无明显降解;在20μL的反应体系中,各成分适宜浓度分别为TaqDNA聚合酶1.0 U、模板DNA 70 ng、dNTPs 250μmol/L、每个特异性引物0.3μmol/L、10×PCR Buffer 2μL,余下的体积用已灭菌的去离子水补充至20μL。In this paper, genomic DNA was isolated by CTAB method using Pyrus ussuriensis as material. The influence of different factors and concentrations in SRAP reaction system on an amplification reaction was discussed. The result showed that the genomic DNA obtained in this experiment was purity and had no significant degradation. In 20 μL reaction system, the suitable concentration was： Taq DNA polymerase 1.0 U, the template DNA 70 ng,dNTPs 250 μmol/L, each of specific primers 0.3 μmol/L,2 μL of 10× PCR Buffer, and sterilized deionized water.

关 键 词：山梨 相关序列扩增多态性分子标记 反应体系 

分 类 号：S661.2[农业科学—果树学] Q781[农业科学—园艺学]