大豆叶绿素含量动态表达的QTL分析  

QTL Analysis for Dynamic Expression of Chlorophyll Content in Soybean (Glycine max L. Merri.)

作　　者：李广军[1,2] 李河南[1] 程利国[1] 章元明[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/国家大豆改良中心,江苏南京210095 [2]临沂师范学院,山东临沂276005

出　　处：《作物学报》2010年第2期242-248,共7页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30470998);教育部新世纪优秀人才支持计划项目(NECT-05-0489);高等学校博士点基金项目(20060307008);江苏省自然科学基金项目(BK2008335);教育部高等学校学科创新引智计划(111计划)(B08025)资助

摘　　要：叶绿素是光合作用中最重要的色素,与大豆籽粒产量密切相关。本研究采用溧水中子黄豆×南农493-1后代衍生的244个F2单株及筛选的150个SSR分子标记构建的连锁遗传图谱,在苗期至开花期测定F2衍生F2:3和F2:4家系生长正常单株的倒3复叶功能叶(非离体)的叶绿素含量13次,通过Windows QTL Cartographer v2.5软件包的复合区间法,动态定位了大豆叶绿素含量的QTL。结果表明,不同时间点共检测到20个QTL,其中,不同发育阶段间、年份间和地点间共同的QTL较少,不同时间点上的QTL差异较大,重复出现在N、D1a、F和K连锁群的QTL有3～4次。这些结果为叶绿素含量的遗传剖析和标记辅助育种提供理论依据。Chlorophyll is the most important photosynthetic pigment and closely related to soybean seed yield. However, there are still very few studies at different developmental stages under multiple environments. A genetic linkage map using 244 F2 plants derived from a soybean cross between Lishuizhongzihuang and Narmong 493-1 was constructed. The F2：3 and F2：4 families were used to dissect the developmental behavior for chlorophyll content across different environments by quantitative trait locus （QTL） mapping approach. Chlorophyll contents for the function leaf （in vivo） in the 244 F2：3 and F2：4 families from seedling to blooming stages at Jiangpu and/or Linyi experimental stations in 2007 and/or 2008 were measured by SPAD-502 chlorophyll instrument. Composite interval mapping （C1M） of Windows QTL Cartographer v2.5 was used for the QTL analysis. A total of 20 QTLs were detected at various developmental stages under the two environments. However, there were few common QTLs identified across different developmental stages, years, and experimental stations, although there were 3 to 4 QTLs detected on each of the N, Dla, F, and K linkage groups. In the two years, one common QTL, qchl-Dla-1, located between markers sat_160 and satt147 on the linkage group Dla, was identified at Jiangpu experimental station. At the two experimental stations above, three common QTLs on the K, M, and N linkage groups were mapped. These results provide a theoretical basis for genetic analysis of chlorophyll traits and marker-assisted breeding.

关 键 词：大豆 叶绿素含量 QTL 动态表达 

分 类 号：S565.1[农业科学—作物学]