一个新矮生玉米种质资源矮生性状QTL的定位  

QTL Identification for Plant Height in a New Dwarf Germplasm of Maize

作　　者：石云素[1] 于永涛[1] 宋燕春[1] 刘志斋[1] 黎裕[1] 王天宇[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《作物学报》2010年第2期256-260,共5页Acta Agronomica Sinica

基　　金：国家科技支撑计划项目(2006BAD13B03);北京市自然科学基金项目(6071003);农作物种质资源保护与利用项目(NB06-070401-22-27-04)资助

摘　　要：用新发现的玉米矮生种质资源矮2003×冀257构建的255个F2:3家系为作图群体,利用114个覆盖玉米全基因组的SSR标记构建连锁图谱,图谱总长度2852.1cM,标记间平均距离为27.42cM。2006年在北京与海南进行随机区组试验,鉴定了255个F2:3家系成株期株高。用复合区间作图法(composite interval mapping,CIM),对控制玉米株高性状的遗传位点进行QTL检测。在两个不同环境下均检测到相同的控制玉米株高的QTL位点3个,分别位于第1和第2条染色体。其中在第1染色体上的1.10～1.11区段存在一个控制株高的主效QTL,与dwarf plant8(d8)位置相近,在北京和海南环境下分别能够解释株高表型变异的50.5%和37.5%,作用方式表现为显性效应。深入的序列分析结果显示,该基因/QTL位于已知的d8基因下游20～30cM的染色体区间,这可能是玉米中控制株高的一个新基因。Dwarf germplasm is important for both breeding programs and basic researches in maize （Zea mays L.）. Previously we found a naturally occurred dwarf mutant from the inbred ＂K36＂ and developed a new dwarf germplasm, ＂Ai 2003＂, with good agronomical performance. Classical genetic analysis showed that this dwarfing character was controlled by a major single recessive nuclear gene. However, the character of plant height in the germplasm is also likely associated with other genetic loci. Therefore, quantitative trait locus （QTL） analysis was conducted to elucidate the genetic basis of plant height of this dwarf germplasm by using a segregating population consisting of 255 F2：3 lines derived from a cross between Ai 2003 and a normal inbred, Ji 257. A genetic linkage map was constructed by 114 polymorphism simple sequence repeat （SSR） markers covering the entire maize genome. The total map length was 2 852.1 cM, with an average distance of 27.4 cM between markers. Composite interval mapping （CIM） was used to identify the genes/QTLs controlled plant height based on the phenotypic characterization of 255 F2：3 families in Beijing and Hainan in 2006. The same three QTLs located on chromosomes 1 and 2 were identified under both environments, explaining 4.8% to 50.5% of the phenotypic variances, among which, one major QTL located in the region of bin 1.10-1.11 explained 50.5 % and 37.5% of the phenotypic variation in B eijing and Hainan, respectively. A further sequences analysis revealed that the QTL is located in the 20-30 cM interval downstream of dwarf plant8 （d8）, a well-known maize dwarf gene, implying that the locus is a gene newly discovered for controlling plant height in maize

关 键 词：玉米 矮生资源 SSR 株高 QTL 

分 类 号：S513[农业科学—作物学]