^(131)I标记抗c-erbB-2单克隆抗体对卵巢癌动物模型的生长抑制作用  

Inhibitory effect of anti-c-erbB-2 monoclonal antibody labeled with ^(131)I on growth of ovarian cancer in mude mice

作　　者：许天敏[1] 崔满华[1] 谷丽萍[1] 崔松花[1] 苏曼曼[2] 王文加[2] 王丁丁[3] 

机构地区：[1]吉林大学第二医院妇产科,吉林长春130041 [2]吉林大学药学院医学生物工程教研室,吉林长春130021 [3]广东药学院生命科学与生物制药学院生物技术教研室,广东广州510240

出　　处：《吉林大学学报（医学版）》2010年第1期63-66,I0001,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200705203;20080134)

摘　　要：目的:用131I标记抗c-erbB-2单克隆抗体,探讨其对荷人卵巢癌裸鼠的生长抑制作用。方法:将抗c-erbB-2单克隆抗体与131I偶联,制成免疫靶向药物,将已接种卵巢癌SKOV3细胞且肿瘤直径已达1.0cm的裸鼠分为131I标记抗c-erbB-2单克隆抗体3.7MBq低剂量组和11.1MBq高剂量组,以131I标记的mIgG(非治疗性抗体)为对照组,对三组荷人卵巢癌裸鼠模型分别进行肿瘤局部注射,于用药当日后6周内每周测量肿瘤体积、质量,计算抑瘤率,并与对照组进行比较。结果:131I标记抗c-erbB-2单克隆抗体3.7MBq低剂量组和11.1MBq高剂量组与对照组比较肿瘤体积减小(P<0.05)、肿瘤质量减轻(P<0.05)、抑瘤率增高(P<0.05),同时131I标记抗c-erbB-2单克隆抗体11.1MBq高剂量组肿瘤体积和肿瘤质量低于3.7MBq低剂量组(P<0.05)、抑瘤率高于3.7MBq低剂量组(P<0.05)。结论:131I标记抗c-erbB-2单克隆抗体对荷人卵巢癌裸鼠肿瘤的生长有明显的抑制作用,且以131I标记抗c-erbB-2单克隆抗体11.1MBq剂量作用强于3.7MBq剂量。Objective To label anti- c-erbB-2 monoclonal antibody with 131I, and investigate its inhibitory effect on the growth of ovarian cancer in mude mice. Methods Anti-c erbB2 monoclonal antibody was combined with ^131I to produce radioimmunological targeting drug. The nude mice which had been inoculated subcutaneously with ovarian cancer SKOV3 cells and the diameter of tumor mass was about 1.0 cm were randomly divided into 3 groups： G1 , G2 and control groups. The mice in G1 and G2 groups were treated with 11.1 MBq and 3.7 MBq anti-c-erbB2 monoclonal antibody labeled with ^131I respectively. The mice in control group was treated with mIgG labeled with ^131I. The size and weight of tumor were determined once every week during 6 weeks after administration. Results Compared with control group, the size and weight of tumor in G1 and G2 groups were significantly decreased, and the inhibitory rate was increased （P〈0.05） . The size and weight of tumor in G1 group were lower than those in G2 group （P〈0. 05）, and the inhibirory rate in G1 group was higher than that in G2 group （P〈 0.05） . Conclusion Anti-e-erbB-2 monoclonal antibody labeled with radionuclide ^131I could inhibit the growth of ovarian cancer in nude mice and the effect of 11.1 MBq c-erbB 2 monoclonal antibody labeled with ^131I is better than 3.7 MBq ^131I c-erbB-2.

关 键 词：卵巢肿瘤 c—erbB-2 抗体 单克隆 ^131I 放射免疫疗法 

分 类 号：R815[医药卫生—放射医学]