左旋精氨酸对糖尿病大鼠的肾脏保护作用  

Protective effect of L-arginine on the kidney of diabetic rats

作　　者：王丽[1] 吴晨光[1] 方春钱[2] 高静[2] 徐志刚[1] 陈艳[1] 陈宇宁[1] 

机构地区：[1]江苏大学附属人民医院内分泌科 [2]江苏大学药学院

出　　处：《江苏大学学报（医学版）》2010年第1期32-35,I0002,共5页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省卫生厅面上项目(H200830);镇江市医学重点人才基金资助项目[镇卫字(2005)237号]

摘　　要：目的:探讨左旋精氨酸(L-arginine,L-Arg)对链脲佐菌素(streptozotocin,STZ)诱导的糖尿病大鼠肾脏保护作用及机制。方法:建立STZ诱导的糖尿病大鼠模型,随机分为糖尿病模型组(DM组)和DM4周后L-Arg治疗组(L-Arg组),并以正常组作对照。观察8周后,测定各组大鼠尿白蛋白排泄率(UAER)、血肌酐(SCr)和血尿素氮(BUN),光镜观察肾组织形态。检测血清和肾皮质中氧化亚氮(NO)、超氧化物歧化酶(SOD)及丙二醛(MDA)含量,测定肾脏线粒体膜电位及肿胀度。结果:与DM组相比,L-Arg组大鼠UAER,SCr,BUN显著降低(P<0.05),肾脏病理形态得到一定改善;血清和肾皮质中NO及SOD含量显著升高(P<0.01,P<0.05),MDA显著降低(P<0.05);肾脏线粒体膜电位显著升高(P<0.05),肿胀度趋势增强。结论:左旋精氨酸对糖尿病大鼠肾脏的保护作用可能与增加NO合成,同时保护肾脏线粒体的功能有关。Objective： To explore the renoprotective effect of L-arginine on streptozotocin-induced diabetic rats and its potential mechanism. Methods： Diabetic models were induced by intraperitoneal injection of streptozotocin STZ in r ＋ ats. Models were randomly divided into two groups ： DM group （ non-treated dia- betic model rats）and L-Arg group（ diabetic model rats treated with L-arginine after 4 weeks ）. The normal non-diabetic rats were as the control group. The excretion of urinary albumin excretion rate（ UAER）, serum creatinine （SCr） and serum urea nitrogen （BUN） were detected after 8 weeks. Morphological changes of kid- ney were observed by optics microscope. The levels of nitric oxide （NO）, the activity of superoxide dismutase （SOD）and malondialdehyde （MDA） in serum and renal cortex were detected;kidney mitochondrial membrane potential and mitochondrial swelling were measured in the different groups. Results： Compared with DM group, the excretion of UAER, SCr and BUN in L-Arg group were significantly decreased（ P 〈 0. 05 ）, while the abnormal pathological changes were improved ; the levels of NO and the activity of SOD in L- Arg group were also significantly increased（P 〈0.01 ,P 〈0.05 ）, the levels of MDA were significantly reduced （ P 〈 0.05 ） ; the kidney mitochondrial membrane potential was significantly elevated （ P 〈 0.05 ） and mitochondrial swelling was reinforced. Conclusion： L-arginine protects the kidneys during diabetic model rats possibly by increasing the levels of NO and improving the function of mitochondria.

关 键 词：左旋精氨酸 糖尿病肾病 氧化亚氮 线粒体 

分 类 号：R587[医药卫生—内分泌] Q244[医药卫生—内科学]