昆布多糖硫酸酯对非激素依赖型人前列腺癌细胞PC-3的作用研究  

Study on Effects of LAMS in Androgen-independentprostate Carcinoma PC-3 Cell Lines

作　　者：邹明畅[1,2] 崔飞伦[1] 盛玉清[1] 蔡宝昌[2] 

机构地区：[1]江苏大学附属人民医院,江苏镇江212002 [2]南京中医药大学,江苏南京210029

出　　处：《现代中药研究与实践》2010年第1期33-36,共4页Research and Practice on Chinese Medicines

基　　金：镇江市科学技术局资助项目(项目编号:SH2007027)

摘　　要：目的研究昆布多糖硫酸酯(LAMS)对体外培养的非激素依赖型人前列腺癌细胞株PC-3的作用。方法利用WST-8法检测LAMS对体外培养的非激素依赖型人前列腺癌细胞株PC-3细胞生长的抑制作用,利用流式细胞术(FCM)分析LAMS对细胞周期和凋亡的影响,利用荧光显微镜观察PC-3细胞形态。结果随着LAMS浓度的增加和作用时间的延长,对非激素依赖型人前列腺癌细胞株PC-3细胞的生长抑制率逐渐增高,呈时间-剂量效应;流式细胞仪分析,PC-3细胞凋亡率逐渐增高;但PC-3细胞的凋亡率与作用时间的延长无明显关系;荧光显微镜观察,PC-3细胞凋亡小体逐渐增多,甚至出现细胞死亡;随着LAMS浓度增加PC-3细胞G1期细胞比例逐渐减少,S+G2期细胞比例呈剂量依赖性增加,表明存在S+G2期阻滞。结论LAMS能抑制体外PC-3细胞的生长,并促进其S+G2期阻滞和凋亡,可能是其抑制前列腺癌的机制之一。Objective To study effects of LAMS on androgen- independent prostate carcinoma PC-3 cell lines in vitro. Methods The inhibitory effects of LAMS on androgen-independent prostate carcinoma PC-3 cells were analyzed by WST-8 assay, cell cycle and apoptosis were analyzed by flow cytometry （FCM） and cell morphology were observed under fluorescence microscope. ResuLts The growth inhibitions of LAMS on androgen-independent prostate carcinoma PC-3 cells were concentration-dependent and time-dependent; apoptosis rate of prostate carcinoma PC-3 cells analyzed by flow cytometry was increased along with the increase of LAMS concentration, however, the resμLts was not concerned with action time; increasing apoptotic bodies and cell death were observed under fluorescence microscope; cell cycle analysis showed PC-3 cells were arrested at S＋G2 phase. This increase in S＋G2 cell popμLation was accompanied with a concomitant decrease of cell number in G1 phase in this cell line. Conclusion LAMS has a significant effect on proliferation inhibition, arrested cell cycle at S＋G2 phase and induce apoptosis of prostate carcinoma PC-3 cells. Those may be one of the mechanisms for LAMS inhibiting prostate carcinoma PC-3 cells.

关 键 词：昆布多糖硫酸酯 PC-3细胞 WST-8 FCM 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]