HPLC法测定人体尿液样品中的4′-羟基黄烷酮和6-甲氧基黄烷酮  

Determination of 4'-hydroxyflavanoneand 6-methoxyflavanone in Human Urine Samples by HPLC

作　　者：何小英[1] 李来生[1] 陈会明[1] 金艳红[1] 

机构地区：[1]南昌大学分析测试中心,江西南昌330031

出　　处：《南昌大学学报（理科版）》2009年第6期565-568,共4页Journal of Nanchang University(Natural Science)

基　　金：江西省教育厅基金资助项目(JJ2007-18)

摘　　要：报道一种快速测定人体尿液中4’-羟基黄烷酮和6-甲氧基黄烷酮的HPLC方法。采用自制的大蒜新素键合硅胶填充柱（DTSP，4．6mmi．d．×15mm，10μm），以乙腈／1．5％三乙胺-甲酸（65：35，v／v，pH=3．0）为流动相，流速设定为1．0mL·minn^-1，检测波长为257nm时，实现了人体尿样品中上述黄烷酮化合物的良好分离。4’-羟基黄烷酮的线性范围为5～100mg·L^-1，r=0．9920；6-甲氧基黄烷酮线性范围为5～100mg·L^-1，r=0．9991，它们的最低检出限分别为1．03ng和1．56ng，平均回收率分别为99．02％～99．26％和98．21％-98．62％，相应的RSD分别小于0．354％和0．605％（n=5）。该方法快速、简便、可靠，适用于人体尿液样品中上述黄烷酮分离分析。A rapid analytical method of determinating 4＇- hydroxyflavanone and 6 - methoxyflavanone in human urine samples by HPLC. Baseline separation was carried out on a home - made allitridin bonded silica gel packed column with acetonitrile/1.5%TEA- HCOOH buffer（65 ： 35, v/v,pH = 3 ）as mobile phase at a flow rate of 1.0 mL· minn ^-1. The detection wavelength was at 257nm. The two kinds of flavanones in human urine were separated The linear ranges and the correlation coefficient of 4＇ -hydroxyflavanone and 6 -methoxylflavanone were 5 - 100 mg ·L^-1 （ r = 0. 9920） and 5 - 100 mg·L^-1, （ r = 0.9991 ）. The average recoveries from spiked human urine samples were 99.02% - 99.26% （ RSD 〈 0. 354%, n = 5） and 98.21% - 98.62% （ RSD 〈 0. 605%, n = 5）, and their minimum detection limit were 1.03 ng, 1.56 ng, respectively. The method is rapid, simple and reliable for the determination of the above flavanones in human urines.

关 键 词：高效液相色谱法 大蒜新素键合硅胶填充柱 黄烷酮化合物 人体尿液样品 

分 类 号：O657.72[理学—分析化学]