秋水仙素诱导野生杜鹃花变异试验  

Experiment on Induction of Three Species of Rhododendron by Colchicines

作　　者：杨华[1] 兰红星 宋绪忠[1] 杜国坚[1] 陈卓梅[1] 

机构地区：[1]浙江省林业科学研究院,浙江杭州310023 [2]浙江省松阳县林业技术推广站,浙江松阳323400

出　　处：《浙江林业科技》2009年第6期53-56,共4页Journal of Zhejiang Forestry Science and Technology

基　　金：浙江省科技计划项目"浙江杜鹃花属植物优良种质筛选及创新利用研究"(2008C32007)

摘　　要：应用0.5、1.0、2.0、3.0 mg/mL 4种浓度的秋水仙素溶液诱导正在发育的映山红(Rhododendron simsii)、马银花(Rh.ovatum)、满山红(Rh.mariesii)的花芽和叶芽,观察其花粉粒及气孔变异情况。结果表明,3种杜鹃叶芽和花芽表现出的多倍性有一定的变化,映山红花粉粒在3.0 mg/mL的浓度处理时变异最大,增加37.99%;满山红花粉粒在2.0 mg/mL的浓度处理时变异最大,增加21.96%;随着秋水仙素浓度的增加,3种杜鹃的气孔器大小变异增加,在3.0 mg/mL浓度处理时增加最多,出现气孔增长、保卫细胞宽增加、保卫细胞长度增加等多倍体表现;气孔密度变化与多倍性联系规律不明显。Induction was conducted on developing flower and leaf bud of Rhododendron simsii, R. ovatum and R. mariesii with four treatments of colchicines concentrations, 0.5mg/mL, 1.0 mg/mL, 2.0 mg/mL and 3.0 mg/mL. Observation on microspore and stomata of tested leaf and flower buds demonstrated polyploid variation. Microspore of R. simsii increased 37.99% with 3.0 mg/mL of colchicines while that of R. mariesii increased 21.96% with 2.0 mg/mL. Stomata of three tested Rhododendrons varied w, ith increase of colchicines concentration topped at 3.0 mg/mL. The variation of stomata density had no evident relation with polyploid

关 键 词：秋水仙素 满山红 映山红 马银花 杜鹃 花粉 气孔 

分 类 号：S685.21[农业科学—观赏园艺]