11个芒果品种SSR指纹图谱的构建与品种鉴别  

Construction of Fingerprinting Map and Identification of Cultivars for 11 Mango Cultivars by SSR Markers

作　　者：姚全胜[1] 詹儒林[1] 黄丽芳[2] 王松标[1] 武红霞[1] 马小卫[1] 邢珊珊[1] 雷新涛[3] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [2]海南大学园艺学院,海南儋州571737 [3]中国热带农业科学院,海南儋州571737

出　　处：《热带作物学报》2009年第11期1572-1576,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.30760147);广东省农业攻关计划项目(No.2005B20901026);农业科技成果转化资金项目(No.2008GB48B06);林业科技支撑计划项目(No.2006BAD01A1705);中央公益性基本科研业务费(No.0062007018);公益性行业科研专项经费项目"芒果规范栽培及冷藏贮藏技术研究"资助

摘　　要：以11个有一定代表性的芒果品种为试材,从16对SSR引物中筛选14对多态性引物构建11个品种的指纹图谱。结果共检测出61个片段,其中多态性片段56个,每对引物可以检测到2～10个数目不等的片段,平均为4.4个,片段大小介于100～540bp之间。11个芒果品种的SSR指纹图谱互不相同,可以作为各品种的特定图谱,单一多态性引物能将11个芒果品种区分为2～5个类型,平均为3.3个类型。14对多态性引物中不同的4对核心引物指纹图谱可以将11个品种区分开。From 16 pairs of SSR primers, 14 pairs were selected to establish DNA fingerprinting maps for 11 representative mango cultivars. A total of 61 bands were detected, of which 56 were polymorphic, and 2 to 10 alleles were detected by each pair of primer, with an average of 4.4. The band size varied from 100 bp to 540 bp. The fingerprinting maps for the 11 cuhivars were different from each other, and 11 cultivars could be divided into 2 to 5 groups by single polymorphism primer, with an average of 3.3. All the 11 cuhivars could be distinguished by the fingerprinting maps constructed by 4 pairs of core primers.

关 键 词：芒果 SSR标记 指纹图谱 品种鉴别 

分 类 号：S667.7[农业科学—果树学]