红将军苹果的脱毒与检测技术研究  

Virus Elimination and Detection Technique in Red General Fuji Apple Cultivar(Malus domestica)

作　　者：苏佳明[1,2] 段小娜[1] 于强[1] 徐维华[1] 姜中武[1] 董雅凤[3] 陈学森[2] 

机构地区：[1]山东省烟台市农业科学研究院,山东烟台265500 [2]山东农业大学园艺科学与工程学院,山东泰安271018 [3]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《华北农学报》2009年第B12期93-96,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家"863"计划重点项目(2006AA100108);山东省科技攻关计划项目(2006GG3209016)

摘　　要：以中熟红富士品种红将军的继代培养4—5代试管苗为试材，进行脱毒处理，并利用ELISA和RT—PCR技术检测脱毒效果。结果表明：红将军苹果试管苗在38℃高温条件下恒定热处理20d，再二次茎尖培养后，试管苗脱毒率达86．4％-100％，可以有效脱除ASSVd、ASGV、ASPV、ApMV和ACLSV等主要的苹果病毒。In vitro seedlings subcultured 4 -5 generation of midseason Fuji apple variety Red General were eliminated viruses,and then were detected by ELISA and RT-PCR. Results showed that keeping constant heat treatment conditions at 38℃ for 20 days,the virus elimination rate of Red General apple reached 86.4% -100% , and major apple viruses such as ASSVd,ASGV,ASPV,ApMV and ACLSV were eluted well.

关 键 词：苹果 脱毒 ELISA RT—PCR 

分 类 号：S661.03[农业科学—果树学]