HMC毒素引起玉米根冠活细胞凋亡的荧光显微观察  

Apoptosis in Detached Corn Root Cap Cells Treated by HMC-toxin Under Fluorescent Microscope

作　　者：马春红[1] 郑秋玲[2] 董文琦[3] 李秀丽[1] 张红心[4] 李运朝[1] 商闯[1] 范尉尉[5] 贾银锁[1] 

机构地区：[1]河北省农林科学院遗传生理研究所,河北石家庄050051 [2]河北省农林科学院滨海农业研究所,河北唐海063200 [3]河北省农林科学院科技处,河北石家庄050051 [4]唐山师范学院生命科学系,河北唐山063000 [5]石家庄市疾病预防控制中心,河北石家庄050011

出　　处：《华北农学报》2009年第B12期135-139,共5页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金项目(C2006000744);科技部国际科技合作项目(2006DFB02480);河北省科学技术研究与发展计划项目(07297162D);农业部"948"项目(2008-Z20)

摘　　要：采用根冠细胞测定法,以玉米小斑病菌C小种毒素(HMC-toxin)处理基因型为B37的同核异质体(Homo-caryon)雄不育C细胞质和正常N细胞质玉米的根冠离体细胞后,采用吖啶橙荧光染色,荧光显微镜下发现HMC毒素可引起细胞凋亡,C细胞质中的凋亡细胞,多于N细胞质中的凋亡细胞,在对照中未观察到凋亡现象。还记载了玉米根冠细胞凋亡的具体过程。Apoptosis in detached corn root cap cells under fluorescent microscope was studied. A pair of homokaryon（ CB37 and B37）inbred lines in maize was tested and the detached corn root cap cells might be using acridine orange staining. Apoptosis was found under fluorescence microscope treated by filtrated culture fluid of HMC toxin. The sensitivity of HMC toxin to C cytoplasm was greater than N cytoplasm, so that the quantity of the apoptosis was great more in the detached corn root cap cells with C cytoplasm than with N cytoplasm. Besides,apoptosis was not found in the CK. These indicated that special disease response occurred in the course of apoptosis in detached corn root cap cells of C cytoplasm maize led by HMC.

关 键 词：玉米小斑病菌C小种 毒素培养滤液 根冠细胞 细胞凋亡 

分 类 号：S513.01[农业科学—作物学]