猪瘟重组蛋白E2的兔体免疫保护试验  

Immunological Analysis of the E2 Glycoprotein of Classical Swine Fever Virus in Rabbits

作　　者：田宏[1] 吴锦艳[1] 尹双辉[1] 尚佑军[1] 郑海学[1] 刘湘涛[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点实验室,甘肃兰州730046

出　　处：《华北农学报》2009年第B12期225-228,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家"863"高技术研究发展计划资助项目(2003AA241110)

摘　　要：鉴于猪瘟病毒不能使家兔发病,但能使之产生免疫原性,而且猪瘟兔化弱度苗能在兔体的定型热反应。本研究选用家兔为试验模型,将自制的亚单位疫苗用弗氏佐剂乳化后免疫家兔,持续一定的免疫周期后,用猪瘟活疫苗(II)攻击免疫及对照试验家兔。根据T淋巴细胞增殖试验、阻断ELISA及攻毒保护试验对所制备的抗原进行了全面的评价。结果表明,该免疫原具有良好的免疫原性,而且能保护免疫家兔为4/4。可以进行下一步的开发和利用价值。以期该疫苗能为猪瘟的防治做出贡献。To investigate the immunogeneicity of a subunit vaccine of E2 glycoprotein of classical swine fever virus（CSFV）. In this study,the rabbits were immunized with the homemade antigen（recombinant E2 protein） ,T- lymphocyte proliferation response, blocking ELISA and virus attacked test were used to detect the effect of the immunized responses in guinea pigs. The results indicated that a retroviral based vaccine carrying the E2 gene of CSF was able to elicit strong CSFV specific humoral immune responses in guinea pigs. These results encourages further work towards the development of a vaccine against CSFV infection.

关 键 词：猪瘟病毒 亚单位疫苗 E2蛋白 免疫试验 

分 类 号：S432[农业科学—植物病理学]