棉花黄枯萎病拮抗菌株的筛选及抗菌蛋白的分离纯化研究  

Study on the Purifying of the Antagonistic Protein and the Isolation of Antagonistic Strain of Cotton Wilt

作　　者：张双凤[1] 张爱民[1] 赵钢勇[1] 张克宏[1] 

机构地区：[1]河北大学生物技术中心河北省生物工程技术研究中心,河北保定071002

出　　处：《华北农学报》2009年第B12期229-232,共4页Acta Agriculturae Boreali-Sinica

摘　　要：从棉花植株根部筛选的108株芽孢杆菌中,通过对棉花黄枯萎主要病原菌大丽轮枝菌拮抗效果的复筛,分离到一株LC-105菌株,其抑菌圈达到20 mm以上;本研究从该菌株的发酵代谢产物中,分离出活性拮抗物质,进一步通过DEAE-Sephadex A-50离子交换层析和Sephacryl-S100凝胶层析对其代谢产物进行了分离纯化,证明该拮抗物质为一种蛋白质,最后通过聚丙烯酰胺凝胶电泳证实了所分离物质的纯度达到电泳纯。108 Bacillus strain has been isolated from the roots of cotton,LC-105 strain has been chosen by the antagonistic effect to the cotton wilt and diameter of the antagonistic circle reach more than 20 mm. Antagonistic material has been separated from the metabolism of the strain. Antagonistic material purifying has been done by DEAE-Sephadex iron-exchange chromatography and Sephacryl-S100 gel chromatography. The antagonistic material is proved to be a kind of protein. Electrophoresis pure has been identified by the polyacrylamide gel electrophoresis method.

关 键 词：棉花 黄枯萎病 拮抗菌株 拮抗物质 分离纯化 凝胶电泳 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]