热休克蛋白70与多环芳烃相关肺癌关系的研究  

Study on relationship between HSP70 and polycyclic aromatic hydrocarbons-related lung cancer

作　　者：蔡元萍[1] 卢桥发[1] 苏艳伟[1] 邬堂春[2] 

机构地区：[1]武汉市普爱医院呼吸内科,湖北武汉430033 [2]华中科技大学同济医学院公共卫生学院,武汉430030

出　　处：《中国职业医学》2009年第6期464-466,共3页China Occupational Medicine

基　　金：国家自然科学基金资助项目(30170799);国家教育部博士点基金课题(20010001092)

摘　　要：目的分析热休克蛋白70抗体(HSP70-Ab)与多环芳烃(PAH)相关肺癌的关系,为PAH相关肺癌发病机制及诊断指标的研究提供参考依据。方法采用Western Blot免疫印迹-酶联免疫吸附测定(ELISA)法分别检测PAH相关肺癌患者(肺癌组,52例)、非肿瘤性肺病者(配比组,34例)和健康者(健康组,30例)血浆中HSP70-Ab的抗体滴度。结果肺癌组不同抗体滴度组阳性检出率显著高于配比组和健康组(均P<0.05),配比组、健康组各滴度组间比较差异无显著性(均P>0.05)。logistic回归分析表明,接触苯并(a)芘[B(a)P]、同时接触粉尘及B(a)P与HSP70-Ab(1∶40)密切相关(分别为OR=5.1074,P=0.0433和OR=2.7874,P=0.0209)。结论HSP70可能与PAH相关肺癌患病有关,血浆HSP70-Ab高滴度(1∶40)可能是PAH相关肺癌的血清学标志物。Objective To explore the relationship between heat shock protein 70 antibody （HSP70-Ab） and polycyelic aromatic hydrocarbons （PAH） -related lung cancer, in order to evaluate its possible role in the genesis of PAHs-related lung cancer,and to investigate the contribution of PAH in clinical diagnosis and treatment. Methods Western blot-ELISA was applied to detect the titer of HSP70-Ab of patients in lung cancer group （ n = 52）, matched group （ n = 34） and healthy group （ n = 30）. Results HSP70-Ab of different titer subsets in lung cancer group was significantly higher than that in matched group and healthy group （ P 〈 0. 05 ）. Meanwhile, there was no significant difference between matched group and healthy group （ P 〉 0. 05）. Logistic analysis indicated that exposure only to B（a） P or to both dust and B（a） P was associated with HSP70-Ab （OR = 2. 787 4, P = 0. 020 9 and OR = 5. 107 4,P = 0. 043 3, respectively）. Conclusion HSP70 might be associated with PAH related lung cancer, and HSP70-Ab at the titer of 1 ： 40 of plasma might serve as a biomarker for PAH-related lung cancer.

关 键 词：癌 肺 热休克蛋白70抗体 多环芳烃 苯并(A)芘 

分 类 号：R135.1[医药卫生—劳动卫生] R135.2[医药卫生—公共卫生与预防医学]