两种免疫方式对欧洲鳗鲡肠上皮内淋巴细胞和杯状细胞的影响  

Effects of two vacination methods on lymphocytes and goblet cells in intestinal epithelium of Anguilla anguilla

作　　者：黄春兰[1,2] 林旋[2] 李艳虹[1] 宋铁英[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003 [2]福建农林大学动物科学学院,福建福州350002

出　　处：《福建农业学报》2009年第6期565-569,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目(2007N0033);农业部公益性农业专项(nyhyzx07-043-15);福建省财政专项-福建省农业科学院科技创新团队建设基金项目(STIF-Y02);科技部科技人员服务企业行动项目(2009GJC40042)

摘　　要：利用嗜水气单胞菌-ISCOMs疫苗口服或注射免疫欧洲鳗鲡,分别于口服或注射免疫后第1、7、14和21 d采集欧洲鳗鲡前、中、后肠,经石蜡制片,HE和AB-PAS染色计数肠上皮内淋巴细胞和杯状细胞数量。结果显示:两种免疫方式均能不同程度影响肠道上皮内淋巴细胞和杯状细胞数量;血清抗体效价检测结果显示,免疫7 d后抗体效价开始升高,且注射免疫组血清效价整体水平高于口服免疫组。Oral or injection immunization with Aeromonas hydrophila - ISCOMs was applied on Anguilla anguilla for the study. The numbers of lymphocytes and goblet cells in the intestinal epithelium of A. anguilla at days 1,7,14 and 21 after the vaccination were determined by using HE and AB - PAS staining. The results showed that the im munization changed the numbers of lymphocytes and goblet cells,and the antibody activity in serum became higher after day 7. It was found that the injection immunization produced a higher antibody activity than the oral application.

关 键 词：口服免疫 注射免疫 淋巴细胞 杯状细胞 

分 类 号：S941.99[农业科学—水产养殖]