中国和巴西临床新生隐球菌格鲁比变种菌株的种群和基因型比较分析  

Multilocus microsatellite typing of clinical Cryptococcus neoformans var.grubii strains from China and Brazil

作　　者：朱键[1] 刘莹[2] 大楠悦子[2] 三上襄[2] 

机构地区：[1]贵阳医学院附属医院消化内科,贵阳550004 [2]日本千叶 日本千叶大学真菌医学研究中心

出　　处：《第三军医大学学报》2010年第3期206-209,共4页Journal of Third Military Medical University

基　　金：日本文部省资助项目(2008)~~

摘　　要：目的了解新生隐球菌格鲁比变种(Cryptococcus neoformans var.grubii)临床菌株在国内和巴西多位点微卫星位点定型(multilocus microsatellite typing MLMT)的种群分布特征,探讨并分析两国新生隐球菌格鲁比变种MLMT基因型差异。方法对已鉴定的69株新生隐球菌格鲁比变种进行DNA提取,采用3个微卫星位点(CNG1、CNG2、CNG3)的正反序列引物对相应的微卫星位点基因片段进行扩增后测序;测序后计算出每一菌株位点基序重复数(CNG1对应TA重复,CNG2对应GA重复,CNG3对应CAT重复);结合不同基序重复数判定各菌株的基因型。应用SPSS 11.5统计软件进行统计学分析。结果在33株被MLMT基因分型的国内新生隐球菌grubii变种菌株中有5种基因型被鉴定,其中29株为MLMT-17,占87.88%;而在36株巴西临床菌株中有10种基因型被鉴定,其中19株为MLMT-13,占52.78%。结论两国间新生隐球菌格鲁比变种临床菌株的主要基因型分布存在显著差异。巴西临床菌株的基因型分布表现为多样性。Objective To understand the genotype distribution of clinical Cryptococcus neoformans vat. grubii strains from China and Brazil using multilocus microsatellite typing （MLMT） method and to study the difference in their MLMT genotyping. Methods DNA was extracted from the identified 69 clinical Cryptococcus neoformans var. grubii strains. DNA fragments covering microsatellite loci （ CNG1, CNG2, and CNG3） were amplified by PCR and sequenced. The number of each motif repeats in 3 microsatellite regions （ ＂TA＂, ＂GA＂, and ＂CAT＂ repeats for CNG1 ,CNG2, and CNG3, respectively） was calculated. The MLMT types of 69 clinical Cryptococcus neoformans var. grubii strains were determined according to the repeat number of different motifs. Data were analyzed using SPSS 11.5 software. Results Five genotypes were identified in 33 clinical strains from China. Of these strains, 29 were MLMT-17, accounting for 87.88% of the total strains. Ten genotypes were identified in 36 clinical strains from Brazil. Of the 36 strains, 19 were MLMT-13, accounting for 52.78% of the total strains. Conclusion The difference is great in major genotype distributions of the clinical Cryptococcus neoformans vat. grubii strains from China and Brazil. The genotype of clinical strains from Brazil is diversely distributed.

关 键 词：新生隐球菌变种格鲁比 临床菌株 多位点微卫星灶分型 

分 类 号：R372[医药卫生—病原生物学] R397.5[医药卫生—基础医学]