异氟烷对大鼠学习记忆功能及海马乙酰胆碱受体表达的影响  

Effect of isoflurane on memory of rats and expression of acetylcholine receptor in hippocampus

作　　者：石胜驰[1] 罗铁山[1] 李鹏[1] 易斌[1] 陶国才[1] 

机构地区：[1]第三军医大学西南医院麻醉科,重庆400038

出　　处：《第三军医大学学报》2010年第4期357-360,共4页Journal of Third Military Medical University

摘　　要：目的观察吸入1.2%异氟烷5h后成年和老年大鼠学习记忆功能的变化及海马α4型烟碱乙酰胆碱受体(alpha 4 nicotine acetylcholine receptor,α4nAChR)表达改变。方法健康成年和老年SD大鼠各50只,分为老年对照组(A0),水迷宫老年对照组(WA0),水迷宫老年异氟烷1(WA1)、3(WA3)、7d组(WA7),成年对照组(B0),水迷宫成年对照组(WB0),水迷宫成年异氟烷1(WB1)、3(WB3)、7d组(WB7),每组10只。WA1、WA3、WA7组和WB1、WB3、WB7组分别吸入1.2%异氟烷5h后与WA0、WB0组水迷宫训练检测学习记忆功能,WA1和WB1组于第1天,WA3和WB3组于第3天,A0、WA0、WA7、B0、WB0和WB7组于第7天处死取海马,Westernblot检测α4nAChR表达,并观察海马免疫反应阳性细胞的分布。结果水迷宫实验表明WA0、WB0组无明显差异,WA7(第3、4、5天)潜伏期与WA0(第3、4、5天)、WB0(第3、4、5天)及WB7组(第3、4、5天)相比明显延长(P<0.05),WA3(第3天)潜伏期与WA0(第3天)、WB0(第3天)及WB7组(第3天)相比明显延长(P<0.05)。WA0、WB0、A0及B0组α4nAChR表达无差异,而WA1、WA3组α4nAChR表达低于WA0、WB0、A0及B0组(P<0.05);同时WA1组较WA0、WB0、A0及B0组α4nAChR免疫反应阳性细胞数明显减少(P<0.05)。结论吸入1.2%异氟烷5h后成年大鼠学习记忆功能无明显改变,而老年大鼠出现下降,可能与海马α4nAChR表达下降有关。Objective To observe the change in memory of aged rats and the expression of alpha 4 acetylcholine receptor in hippocampus 5 h after inhalation of 1.2% isoflurane. Methods Fifty healthy adult and 50 aged SD rats were randomly divided into aged normal control group （A0）, aged water labyrinth normal control group （ WA0）, 1 day isoflurane aged water labyrinth group （ WA1 ）, 3-day isoflurane aged water labyrinth group （WA3）, 7-day isoflurane aged water labyrinth group （WA7）, adult normal control group （B0）, adult water labyrinth normal control group （WB0）, adult 1 day isoflurane water labyrinth group （WB1）, 3-day isoflurane water labyrinth group （WB3）, and 7-day isoflurane water labyrinth group （WB7）, 10 rats in each group. Memory of rats in WA1, WA3, WA7 groups was detected 5 h after inhalation of 1.2% isoflurane while memory in rats of WB1, WB3 and WB7 groups was detected during the Morris water maze testing. The rats in A0, WA0, WA7, B0, WB0 and WB7 groups were killed on day 7 to detect the expression of α4 acetylcholine receptor by Westernblotting for observation of changes in distribution of positive immune reaction cells in hippocampus. Results Water maze test showed that there was no significant difference in expression of alpha 4 acetylcholine receptor between WA0 and WB0 groups. However, the expression level of alpha 4 acetylcholine receptor was significantly lower in latent period of WA1 than in latent period of WA0, WB0 and WB7 groups （P 〈 0. 05 ）. A significant change was observed in latent period of WA3 compared with WA0, WB0 and WB7 groups （ P 〈 0. 05 ）. Western blot analysis showed that the expression level of alpha 4 acetylcholine receptor was not significantly different WA0, WB0, A0 and B0 groups （ P 〈 0.05 ）. However, the expression of alpha 4 acetylcholine receptor was lower in WA1 （2.18 ±0.12） and WA3（2.21 ±0. ll）than in WA0, WB0, A0 and B0 groups. The number of immune positive cells was not significantly different in WA0, WB0, A

关 键 词：异氟醚 麻醉药 吸入海马 受体 胆碱能 迷宫学习 记忆 大鼠 

分 类 号：R338.26[医药卫生—人体生理学] R338.64[医药卫生—基础医学]