风疹病毒感染人胚晶状体上皮细胞对热休克蛋白及其转录因子表达的影响  

The effects of rubella virus R16 infection on heat shock protein 70 and heat shock transcription factor 4 in the human embryonic lens epithelial cells in vitro

作　　者：邹俊[1] 卢奕[2] 褚仁远[2] 

机构地区：[1]上海交通大学附属上海第六人民医院眼科,上海200233 [2]复旦大学附属眼耳鼻喉科医院眼科,上海200031

出　　处：《复旦学报（医学版）》2010年第1期6-10,共5页Fudan University Journal of Medical Sciences

基　　金：上海市自然科学基金项目(044119659)

摘　　要：目的观察风疹病毒R16株体外感染人胚晶状体上皮细胞后对热休克蛋白及其转录因子表达的影响。方法风疹病毒R16株体外感染人胚晶状体上皮细胞后3、7、14 d,采用半定量Real-time PCR法检测受风疹病毒R16株感染的人胚晶状体上皮细胞热休克蛋白(heat shock protein 70,HSP70)及其转录因子(heat shock transcription factor 4,HSF4)的mRNA表达水平;Western blot方法检测HSP70的表达;利用DNA测序的方法检测病毒感染的人胚晶状体上皮细胞HSF4的DNA序列变化。结果风疹病毒R16株体外感染人胚晶状体上皮细胞后3、7 d的细胞内HSP70 mRNA和HSP70蛋白水平有所增高,而热休克蛋白转录因子HSF4的mRNA水平有所下降。病毒感染人胚晶状体上皮细胞14 d的细胞DNA中HSF4序列无明显变化。结论风疹病毒R16株体外感染人胚晶状体上皮细胞可以直接诱导HSP70的表达增加,可能在保护细胞免受病毒感染损伤方面具有一定意义。热休克蛋白转录因子HSF4在转录水平对其有负调控作用,但是人胚晶状体上皮细胞受风疹病毒感染后的14 d内其细胞中HSF4的DNA序列不受影响。Objective To investigate the effects of rubella virus R16 infection on heat shock protein 70（HSP70） and heat shock transcription factor 4（HSF4） in the human embryonic lens epithelial cells in vitro. Methods The human embryonic lens epithelial cells were infected by rubella virus R16 for 3,7 and 14 days respectively in vitro.Then the mRNA levels of HSP70 and HSF4 were measured by Real-time PCR assays,the HSP70 protein level was detected by Western blot assay,and the DNA sequence of HSF4 was also identified by DNA Sequencing. Results Both mRNA and protein levels of HSP70 were increased dramatically at 3 and 7 days after rubella virus R16 infection in vitro.But mRNA levels of HSF4 were decreased significantly.The DNA sequence of HSF4 had no change in the human embryonic lens epithelial cells at 14 d after rubella virus R16 infection. Conclusions Rubella virus R16 could directly induce the increased expression of HSP70 in the human embryonic lens epithelial cells infected by rubella virus R16 in vitro,which may prevent human embryonic lens epithelial cells from infecting with virus.The heat shock transcription factor may serve as a negative regulator at transcription level.However,the DNA sequence of HSF4 had no change in the human embryonic lens epithelial cells within 14 days after rubella virus R16 infection.

关 键 词：风疹病毒 人胚晶状体上皮细胞 热休克蛋白 热休克蛋白转录因子 

分 类 号：R339.11[医药卫生—人体生理学]