参附注射液抑制兔再灌注肺组织NF-κB表达的实验研究  

The study of inhibitive effects of Shen-fu injection on NF-κB expression in ischemia-reperfusion rabbit lung

作　　者：邵丰[1] 郑世营[2] 杨如松[1] 许栋生[1] 邹卫[1] 陈静瑜[3] 张晓膺[4] 

机构地区：[1]南京市胸科医院胸外科,南京210029 [2]苏州大学附属第一医院胸心外科,江苏苏州215006 [3]江南大学附属无锡第五人民医院,江苏无锡214007 [4]苏州大学附属第三医院胸心外科,江苏常州213003

出　　处：《临床肺科杂志》2010年第3期305-307,共3页Journal of Clinical Pulmonary Medicine

基　　金：中华人民共和国卫生部科研招标课题资助项目(No200308);江苏省卫生厅医学科技基金重点课题招标项目(NoH200205)

摘　　要：目的初步探讨参附注射液改善兔肺保存效果及可能的作用机制。方法16只健康家兔随机分为两组,对照组肺加入棉子糖(30mmol/L)的改良低钾右旋糖酐(LPD)液灌注及保存,实验组则将参附注射液(40mg/L)加入改良LPD液灌注及保存。在4℃保存12h后再灌注1h,测定动脉血氧分压(PaO2)、肺组织的干湿比(W/D)、肺组织中髓过氧化物酶(MPO)活性、NF-κB的表达并观察肺组织病理结构的变化。结果再灌注15min后,两组的PaO2逐渐降低,但实验组PaO2的降低程度明显低于对照组(P<0.01);再灌注后,实验组的W/D和MPO也明显低于对照组(P<0.01),肺组织NF-κB的表达也明显低于对照组(P<0.01),肺组织病理变化较对照组轻。结论参附注射液可抑制再灌注肺组织中NF-κB的表达,减轻肺缺血再灌注损伤,改善肺功能,对兔肺具有保护作用。Objective To investigate the protective effects of shen-fu injection on rabbit lung during ischemia-reperfusion, and to explore its possible mechanism. Methods Sixteen rabbits were randomly divided into two groups： the control group and the trail group. The harvested lung blocks were flushed with and stored in the modified low-postasssium-dextron （LPD） solution in control group. But in the trail group LPD containing shen-fu injection （40 mg/L） was used. The lung was stored at 4℃ for 12 h in both groups. The isolated rabbit lung reperfusion models were established and the lungs were perfused for 1 h. PaO2 was measured at every 15 min intervals during reperfusion. After reperfusion, the lung tissue wet-to-dry （W/D） ratio and myeloperoxidase （MPO） activity were obtained. The protein expression of nuclear factor-kappa B （NF-KB） was also detected by using immunohistochemistry at the end of reperfusion. Light microscopic and uhrastroctural changes were also examined. Results The level of decreased PaO2 in the trail group were lower than in the control group at the every interval time in the samples obtained 15 min after reperfusion （P 〈0. 01 ）. After reperfusion the W/D, MPO, and the protein expression of NF-KB decreased evidently in the trail group （ P 〈0. 01 ）. The morphologic and uhrastructual damages in the trail group were milder than in the control group. Conclusion Shen-fu injection can inhibit NF-kB expression in ischemia-reperfusion lung, alleviate the ischemia - reperfusion injury of lung and improve the protective effect of lung preservation.

关 键 词：参附 NF-ΚB 肺保护 缺血再灌注损伤 

分 类 号：R285.5[医药卫生—中药学]