反相高效液相色谱法测定血府逐瘀口服液中柚皮苷含量  

Determination of Naringin from Xuefu Zhuyu Oral Liquid by RP-HPLC

作　　者：胡安青 

机构地区：[1]江苏省镇江药品检验所,江苏镇江212003

出　　处：《中国药业》2010年第4期29-29,共1页China Pharmaceuticals

摘　　要：目的建立测定血府逐瘀口服液中柚皮苷含量的反相高效液相色谱(RP-HPLC)法。方法采用十八烷基硅烷键合硅胶柱,流动相为乙腈-水(21∶81),检测波长为283nm,流速为1.0mL/min。结果柚皮苷质量浓度线性范围为10.05～50.25μg/mL,平均回收率为98.45%,RSD为0.73%(n=6)。结论RP-HPLC法简便、快速,结果准确,能有效控制血府逐瘀口服液中的质量。Objective To determine the content of naringin in Xuefu Zhuyu Oral Liquid by RP- HPLC. Methods The determination was carried out with Symmetry C18 column. The mobile phase consisted of acetonitrile-water（21：81）. The flow rate was 1.0 mL/min and the detective wavelength was at 283 nm. Results The linear concentration range of this compound was 10. 05-50.25 ug/mL. The average recovery rate was 98.45%, RSD was 0.73% （n=6）. Conclusion The method is simple and accurate, which can be used to control the quality of Xuefu Zhuyu Oral Liquid.

关 键 词：血府逐瘀口服液 柚皮苷 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]