提高乡镇医院乙肝病毒血清标志物检测质量的研究  

Study on improving the quality for detection of serum markers of hepatitis B virus in township hospitals.

作　　者：黄学斌[1] 陈华根[1] 许颖[2] 宋强[1] 陈宇宁[1] 江俊[2] 

机构地区：[1]成都市新都区人民医院检验科,四川成都610500 [2]成都医学院第一附属医院检验科,四川成都610500

出　　处：《临床和实验医学杂志》2010年第4期261-262,264,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的针对成都市新都区乡镇医院乙肝病毒血清标志物(HBV-M)检测质量问题,采用一系列方法,提高检测质量。方法首先通过对乡镇医院HBV-M检测现状的摸底调查,包括相关设施设备,实验人员学历、职称、所学专业,以及对HBV-M及其检测技术认知情况,发放HBV-M定值血清,评价检测结果准确度等方式,了解乡镇医院HBV-M检测现状。继而采取集中培训、现场指导、组织参加室间质量评价活动等形式,帮助建立质量控制体系、规范操作技术、提高检测水平。最后通过理论考核、参加HBV-M检测室间质评回报成绩、现场考查质量改进情况等方法,评价乡镇医院HBV-M检测质量。结果在设施设备方面,无酶标仪和洗板机的医院拟将添置两种设备,实验所需温度计通过比校皆合格并将按规定送计监部门检定,移液器全部校准;实验人员对HBV-M及其检测技术的认知情况,了解率从50.2%上升到95.3%;质量管理制度从没有到全部建立实施,均能查见质量管理制度、标准操作规程和室内质控记录;参加室间质评能力测试(PT)成绩从78%上升到94.7%。结论通过人员培训、现场指导和参加室间质量评价活动,可以显著提高乡镇医院实验人员HBV-M检测水平,保证检验质量,以满足临床诊治疾病的需要。Objective To explore a series of methods to improve the quality for detection of hepatitis B virus markets （ HBV - M ） in serum for consideration of quality problems remaining in township hospitals, Xindu District of Chengdu. Methods Firstly, investigations were made to understand the present HBV - M detection status in township hospitals including related facilities and equipment, qualification of personnel, job title, specialties, as well as professional knowledge about detection of HBV - M, significance of HBV - M in serum, accuracy of assessed results, etc. Secondly, adoption of collective training, on -site guidance and participating in quality assessment activities were taken to establish a quality control system for standardization of operating techniques and improving the detection level. Finally, assessment of quality in HBV - M detection in township hospitals was made according to achievement in theoretical examinations and in detection of HBV - M and on - site investigation of quality improvement. Results In terms of facilities and equipment, the hospitals should be equipped with ELISA reader and mieroplate washer, all laboratory thermometers should be measured up and sent for measuring in department of quality supervisor; all transfen＇ing pipettes were calibrated. The rate of understanding HBV - M and detection technique of laboratoW operators had been raised from 50.2 % to 95.3 %. The quality management system which had never been established was built up and implemented in all hospitals. The quality management system, standard operating procedures and internal quality control records were able to be followed up. The rate of passing external quality assessment testing （PT） had been raised from 78% to 94.7%. Conclusion Staff training, on - site guidance and participating in external quality assessment activities can significantly improve the technique for detection of HBV - M and detection quality in township hospitals will be met the needs of clinical diagnosis and treatment of diseases.

关 键 词：医院 乙肝病毒血清标志物 检测 质量 

分 类 号：R512.62[医药卫生—内科学] R446.6[医药卫生—临床医学]