利用原子力显微镜观察芥蓝叶片气孔  

Morphological observation on stomata of cabbage mustard by atomic force microscope

作　　者：陈少雄[1] 陈家楠[2] 蔡继业[2] 

机构地区：[1]揭阳职业技术学院生物系,广东揭阳522000 [2]暨南大学化学系,广东广州510632

出　　处：《湖南农业大学学报（自然科学版）》2010年第1期9-11,共3页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(30872404)

摘　　要：利用原子力显微镜(atomic force microscope,AFM)的振荡模式研究芥蓝叶片上表皮气孔的三维形貌,并对气孔在叶片失水过程中的形态变化进行动态观察.结果表明,通过AFM的扫描成像,观察到芥蓝叶片表皮的气孔及其周围的细胞,包括保卫细胞、副卫细胞、其他表皮细胞等的微观结构,还观测到覆盖在副卫细胞的微纤维,测量出这些微纤维的直径约为400nm,间隔约为200nm.The three-dimensional morphology was studied and the dynamic morphological changes in the process of losing water of stomata on the leaf of cabbage mustard were observed by using the tapping mode of atomic force microscope （AFM）. The results showed that stomata and the other surrounding cells, including guard cells, subsidiary cells, and epidermal cells, could be clearly imaged by AFM. Furthermore, the micro fibers was found covering the subsidiary cells with diameter of about 400 nm with interval about 200 nm.

关 键 词：芥蓝叶片气孔 原子力显微镜 形态学 

分 类 号：Q24[生物学—细胞生物学]