树脂原位吸附提高酒明串珠菌不对称合成(R)-2-辛醇的催化效率  

Enhancement of efficiency of asymmetric synthesis of (R)-2-octanol with Oenococcus oeni by in situ absorption of resins

作　　者：孟凡涛[1] 徐岩 

机构地区：[1]江南大学生物工程学院,教育部工业微生物重点实验室,江苏无锡214122

出　　处：《化工进展》2010年第2期319-322,共4页Chemical Industry and Engineering Progress

基　　金：国家重点基础研究发展计划(国家973计划,2003CB716008)

摘　　要：通过运用原位吸附技术构建水/树脂反应系统,利用树脂的原位吸附来提高酒明串珠菌Oenococcus oeni CECT 4730不对称合成(R)-2-辛醇的催化效率。通过比较6种不同型号的吸附树脂对反应的影响,选出了最适的树脂AB-8。通过考察树脂的用量及水/树脂相中底物浓度对反应的影响,发现反应体系中加入一定量合适的吸附树脂,能够显著减小底物和产物的抑制,大幅度的提高初始底物浓度和反应产率。AB-8树脂加量为15g/L的水/树脂系统中,底物浓度为30g/L时,反应产率达到58%,较之水相系统提高了1.7倍。To enhance efficiency of asymmetric synthesis of （R）-2-octanonol with Oenococcus oeni CECT 4730,we applied in situ absorption technique to compose an aqueous/resin system for the reaction. The most suitable resin AB-8 was selected from 6 types of absorption resins with respect to the effect on the bioreduction. After evaluated effect of the load of resin and the substrate concentration in the aqueous/resin system,we found that a suitable aqueous/resin system with a matched load of resin can relieve substrate and product inhibition significantly and elevated the initial substrate concentration and product yield markedly. In an aqueous/resin system of 15 g/L resin AB-8,product yield could reach 58 % at the substrate concentration of 30 g/L with an 1.7 fold increase compared with the aqueous system.

关 键 词：吸附树脂 原位分离 酒明串珠菌 不对称还原 (R)-2-辛醇 

分 类 号：Q814[生物学—生物工程]