体积排阻高效液相色谱-电感耦合等离子体质谱法同时测定印度芥菜(Brassica Juncea)中镉铜锌形态  

Speciation Analysis of Cadmium,Copper and Zinc in Indian Mustard(Brassica Juncea) by Size Exclusion-High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry

作　　者：杨红霞[1] 刘崴[1] 李冰[1] 魏巍[2] 张惠娟[2] 陈登云[3] 

机构地区：[1]国家地质实验测试中心,北京100037 [2]南京农业大学,江苏南京230026 [3]安捷伦科技有限公司,北京100022

出　　处：《岩矿测试》2010年第1期9-13,共5页Rock and Mineral Analysis

基　　金：国家地质实验测试中心基本科研业务费项目资助(200607CSJ02);国土资源部公益性行业科研专项资助(200911043)

摘　　要：建立了体积排阻高效液相色谱-电感耦合等离子体质谱法同时测定印度芥菜中镉、铜、锌形态分析方法。为了防止巯基化合物氧化反应的发生,样品采取液氮保护并在-70℃保存,样品分析全流程采取氮吹防氧化措施。在镉、铜、锌胁迫下,诱导产生植物螯合肽(PCs)。在叶片和根部均检测到3种元素的4种形态,植物螯合肽(PC)3-Cd(Cu,Zn)、植物螯合肽(PC)2-Cd(Cu,Zn)、谷胱甘肽(GSH)-Cd(Cu,Zn)、半胱胺酸(Cys)-Cd(Cu,Zn)及其在植物体内的分布规律。结合植物不同部位Cd、Cu、Zn分布规律初步推断,Cd、Cu、Zn在与GSH及Cys的结合上存在竞争。A method based on the coupling of size-exclusion HPLC with ICP-MS was used for speciation analysis of Cd, Cu and Zn binding non-protein thiols in extracts of Indian mustard （Brassica Juncea）. In the presence of Cd, Cu and Zn stress, phytochelatins （PCs） were induced and metal ions in extracts were associated with the induced PCs. Four different species of （PC）3-Cd（Cu,Zn）, （PC）E-Cd（Cu,Zn）, GSH-Cd（Cu,Zn）, Cys-Cd（Cu,Zn） were detected in stems and roots, and their distribution patterns within plants was studied. The results indicated that there was a competition on the combination of Cd, Cu, Zn with GSH and Cys. A non-oxidizing （nitrogen） sample preparation environment and - 70 ℃ preservation condition were employed to avoid the oxidation of sulfhydryl groups. This research may be useful for understanding transportation of heavy metals in hyperaccumulator.

关 键 词：形态分析 镉 铜 锌 印度芥菜 体积排阻高效液相色谱-电感耦合等离子体质谱法 

分 类 号：O655.6[理学—分析化学] O657.62[理学—化学]