壳聚糖荧光标记反应研究  

Study on label reaction of chitosan with fluorescein

作　　者：范妮[1] 薛伟明[2] 

机构地区：[1]陕西教育学院化学系,陕西西安710061 [2]西北大学化工学院,陕西西安710069

出　　处：《云南大学学报（自然科学版）》2010年第1期77-81,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(50373046)

摘　　要：以壳聚糖-海藻酸钙微球在体检测为研究背景,建立壳聚糖的荧光标记方法.以荧光素异硫氰酸酯(FITC)作为标记化合物,采用紫外-分光光度计在447nm下检测标记在壳聚糖上的FITC,通过m(FITC,μg)/m(壳聚糖,g)表征壳聚糖的荧光标记效率.考察壳聚糖溶液pH值、浓度、反应时间以及壳聚糖分子量、脱乙酰度对壳聚糖荧光标记反应的影响.结果表明:选取相对分子质量20万、脱乙酰度95%的壳聚糖配制成0.01g/mL的溶液,在pH10的条件下与荧光素反应5h将制得标记效率为117.4μg/g的荧光壳聚糖.建立了一种稳定有效的壳聚糖荧光标记方法,为荧光标记壳聚糖-海藻酸钙微球提供了技术支持.A effective and stable method to label chitosan with fluorescein isothiocyanate （FITC） was established in order to detect chitosan - calcium alginate microspheres in vivo. The fluorescence intensity of FITC labeled chitosan was detected at 447 nm by UV - spectrophotometer and the efficiency of FITC label chitosan was characterized by mFTTC（μg）/mchitosan（g）. It was studied that the effect of pH, concentration, reaction time, the molecu- lar weight and deacetylation degree of chitosan on label reaction. The content of FITC on chitosan was determined to be 117.4μg/g after reaction for 5hrs with 0.01 g/mL chitosan （200000 dalton,degree of deacytylation 95% ） at pH 10.

关 键 词：荧光素 标记 壳聚糖 

分 类 号：TQ46[化学工程—制药化工]