汉江上游鲫鱼RAPD遗传多样性研究  

Genetic Diversity of RAPD in Wild Carassius in Upstream of Hanjiang River

作　　者：张涛[1] 路宏朝[1] 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723000

出　　处：《贵州农业科学》2010年第1期116-118,共3页Guizhou Agricultural Sciences

基　　金：陕西省教育厅科研项目"宁强矮马遗传多样性及生长基因(GH)的研究"(07JK212);"微卫星进化属性与功能的比较基因组学研究"(06JK329);陕西理工学院科研项目"宁强矮马遗传多样性及生长激素基因(GH)的研究"(SLGQD0513)

摘　　要：采用RAPD技术从26个随机引物中筛选出8个引物对汉江上游汉中段野生鲫鱼群体的遗传多样性进行研究，结果共检测出512个位点，其中多态位点347个，其多态位点比例（P）为67．77％；利用POPGEN软件获得汉江上游汉中段野生鲫鱼群体11个个体间的遗传距离，个体间遗传距离（D）在0．0086～0．5350，个体间遗传相似系数（S）在0．5856～0．9915，Shannon遗传多样性指数（H0）为0．6525。试验表明，汉江上游汉中段野生鲫鱼群体仍保持着较丰富的遗传多样性，应加以保护。Random amplified polymorphic DNA （RAPD） technique was applied to detect the DNA polymorphism in 11 individuals of wild Carassius from upstream of Hanjiang River. Eight random primers were used and 512 sites were generated, and there were 347 polymorphic loci among them, which with percentage of 67.77%. The genetic distance matrix among the 11 individuals of wild Carassius in upstream of Hanjiang River were calculated by using software POPGEN, the genetic distance （D）, genetic similarity （S）, and Shannon＇s genetic diversity index （H0） among the individuals were 0. 0086-0. 5350,0. 0086- 0. 5350, 0. 652 5 respectively. Result showed that the genetic diversity of wide population Carassius in upstream of Hanjiang River was abundant, it should be protected.

关 键 词：鲫鱼 遗传多样性 随机扩增多态DNA 

分 类 号：S965.117[农业科学—水产养殖]