补肾活血中药对大鼠慢性高眼压模型视网膜神经节细胞凋亡相关基因Bcl-2、Bax表达的影响  

The Effect of Traditional Chinese Medicine (TCM) of BuShenHuoXue Principle on Retinal Ganglion Cells (RGCs) Apoptosis-related Gene Bcl-2,Bax Expression in Rat Model of Elevated Intraocular Pressure (EIOP)

作　　者：李翔[1] 曹水清[1] 毛欣[1] 黄江丽[1] 

机构地区：[1]成都中医药大学附属医院眼科,四川成都610072

出　　处：《四川中医》2010年第2期22-24,共3页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:观察补肾活血中药对SD大鼠慢性高眼压(elevated intraocular pressure,EIOP)模型视网膜神经节细胞凋亡相关基因Bcl-2、Bax表达的影响。方法:采用烙闭上巩膜静脉法,制作大鼠慢性EIOP模型,随机分为模型对照组,补肾活血高剂量组、中剂量组、低剂量组,空白对照组。连续灌胃8周,并于8周末处死大鼠,观察其对EIOP大鼠视网膜神经节细胞(retinal ganglion cells,RGCs)凋亡基因的影响。结果:补肾活血中药可上调大鼠EIOP视网膜神经节细胞抗凋亡基因Bcl-2、抑制凋亡促进基因Bax。结论:补肾活血中药可调控大鼠EIOP视网膜神经节细胞凋亡基因的表达。Objectives： To observe the effect of Traditional Chinese Medicine （TCM） of BuShenHuoXue Principle on retinal ganglion cells （RGCs） apoptosis - related gene Bcl - 2, Bax expression and in rat model of elevated intraocular pressure （ EIOP） . Methods： By unilaterally cauterizing 3 episcleral vessels, the rat model of EIOP was gotten. Rats were divided into 5 groups randomly： model control group, treatment groups with high, middle, low dosage of BuShenHuoXue formula of TCM, and normal control group. After given drugs or normal saline for 8 weeks, the rats were put to death and eyeballs were taken out. To observe the effect ti intraocular pressure （ IOP）, retinal ganglion cells （RGCs） of apoptosis gene. Results： BuShen- HuoXue formula of TCM can increase Bcl-2 gene, degradeBax gene expression, and add RGCsBcl -2/Bax. Conclusions： BuShenHuoXue formula of TCM can regulate the expression of RGCs apoptosis gene.

关 键 词：补肾活血中药 视网膜神经节细胞 凋亡 Bcl-2 BAX 

分 类 号：R259[医药卫生—中西医结合]