白花蛇舌草水提取物对多药耐药白血病细胞HL-60/ADR的作用  

Study the Inhibitory Action and Inducing Apoptosis of Aqueous Extract from Hedyotic Diffusa on Multidrug Resistance Leukemic Cell Line HL-60/ADR

作　　者：赵诗云[1] 尹小明[2] 吴东风[1] 陈厚爱[1] 孙敬[2] 曾黎峰[2] 熊美丽[2] 伍柏青[2] 龚旭疆[2] 申细保[2] 

机构地区：[1]江西省中医药研究院,江西南昌330077 [2]江西省人民医院,江西南昌330006

出　　处：《四川中医》2010年第2期55-57,共3页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:研究白花蛇舌草水提取物对多药耐药白血病细胞HL-60/ADR的抑制作用及机制。方法:采用MTT比色试验观察白花蛇舌草水提取物对多药耐药细胞HL-60/ADR的抑制作用。采用光镜、电镜技术观察细胞形态结构的改变,利用流式细胞术(FCM)检测细胞凋亡率。结果:①白花蛇舌草水提取物能明显抑制HL-60/ADR细胞的生长。②形态学改变,呈典型的凋亡特征。③流式细胞术证实,凋亡率也呈时间和剂量的依赖性。结论:白花蛇舌草水提取物对多药耐药细胞HL-60/ADR的生长具有极强的抑制作用,诱导其凋亡是其主要机制。Objectives ： To study the inhibitory action and mechanism of aqueous extract from Hedyotic Diffusa on multidrug resistance leukemic cell line HL - 60/ADR. Methods ： To observe the depressant effect of aqueous extract from Hedyotic Diffusa on muhidrug structure by light microscope and electron microscope, to detect the apoptosis rate by Flow Cytometry （ FCM ） Results： （1）Aqueous extract from Hedyotic Diffusa could effectively inhibit the proliferation of HL -60/ADR ceils. （2）The morphological changes are the typical character of apoptosis. （3）The apoptotic rate has dependence of dosage and time have been confirmed by Flow Cytometry （ FCM ） . Conclusions ： Aqueous extracts from Hedyotic Diffusa could effectively inhibit the proliferation of multidrug resistance leukemic cell line HL - 60/ADR, induce HL - 60/ADR to apoptosis is one of its mechanism.

关 键 词：白花蛇舌草水提取物 多药耐药 HL-60/ADR 凋亡 

分 类 号：R285.5[医药卫生—中药学]