广东省稻瘟病菌DNA指纹分析及谱型结构  

FINGERPRINTING ANALYSIS OF MAGNAPORTHE GRISEA IN GUANGDONG PROVINCE AND THEIR GENETIC LINEAGE STRUCTURE

作　　者：伍尚忠[1] 罗林[2] 张少红 杨祁云[1] 朱小源[1] 刘斌 

机构地区：[1]广东省农业科学院植保所,广州510640 [2]广东省农科院水稻细胞工程育种重点实验室,广州510640

出　　处：《植物病理学报》1998年第4期323-330,共8页Acta Phytopathologica Sinica

基　　金：广东省自然科学基金;农业部"九五"重点高新技术与基础研究基金;广东省农业科学院院长科技基金

摘　　要：利用散布性重复序列（dispersedrepetitivesequence）MGR586探针与EcoRI组合，分析了采自广东省4个自然生态稻作区的112个猪瘟病菌株的限制性片断长度多态性（RFLPs），根据彼此间RFLPs单型（haplotype）的带型位置相似率达80％为度，把这些菌株划分为15个遗传宗谱（geneticlineage），其中宗谱1及宗谱2占总数的78．58％，遍布全省各地，是优势宗谱。供测菌株的RFLPs单型的多样性显示出我省稻瘟病菌的群体结构呈现多样性。参试菌株的群体遗传多样性值为0．64。谱型分析表明，我省多数病原型是杂合群体。在分子水平上划分的病菌宗谱与寄生品种的遗传背景有密切关系。研究结果也初步建立了病菌宗谱与用以鉴别寄主反应划分的生理小种致病谱型的相互关系。The polymorphism restriction fragment length of (RFLPs) among 112 rice blast pathogen lsolates (Magnaporthe grisea ) collected from the four rice ecological growth regions (RER) in Guangdong was analyzed by dispersed repetitive sequence probe MGR586 and restriction endonuclease EcoRI combination. The total sum of 112 Pathogens was clustered into 15 genetic lineages according to their band patterns in the RFLPs haplotype.Lineage I and lineage 2 may be designated as the predomlnant lineages according to their widely distribution overall the four rice ecological growth region in the province with the total sum 85 out of 112, The genetic diversity value of the pathogen population for testing was 0. 64.Diversity of blast pathogens was closely correlated with the host genetic background,and the relationship between genetic lineage and pathogenicity spectrum was also discussed.

关 键 词：稻瘟病菌 RFLP DNA指纹分析 谱型结构 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]