水稻恶苗病菌的遗传多样性研究  

Genetic diversity in Gibberella fujikuroi (Fusarium section Liseola) from rice.

作　　者：章初龙[1] 郑重[1] 王拱辰[1] 

机构地区：[1]浙江农业大学植保系

出　　处：《浙江农业大学学报》1998年第6期583-586,共4页

基　　金：国家自然科学基金

摘　　要：从浙江省、黑龙江省和上海市分别采集分离到的水稻恶苗病菌菌株分别为３４个、２个和１个，用生物学种（配合群）和营养体亲和群方法分析其遗传多样性．结果表明，有３５个菌株分属于Ａ、Ｄ、Ｅ、Ｆ四个配合群，其中以Ｄ配合群为主，另有２个菌株不能归属于已知的配合群．在这３５个配合群的菌株中，２５个菌株是异核体自身亲和的，且可分为２０个营养体亲和群．其中１７个营养体亲和群分别只含１个菌株，有１个营养体亲和群含４个菌株，有２个营养体亲和群分别含２个菌株．Ｄ、Ｅ、Ｆ三个配合群的遗传多样性指数分别为０．７、０．８和１．０．由此可见水稻恶苗病菌在遗传上的多样性．Thirty seven isolates of Fusarium section Liseola collected from rice with bakanae disease were tested to determine the biological species (mating population) and vegetative compatibility group (VCG) to which they belonged. Among them, 34 isolates were from Zhejiang Province, 2 from Heilongjiang Province, 1 from Shanghai. The sexual crosses with the standard testers from six (A￣F) distinct mating populations of Gibberella fujikuroi indicated that 35 isolates belonged to the 'A', 'D', 'E' and 'F' mating populations,respectively ,with 'D' mating population predominant. Two isolates could not be crossed with any of the known mating populations. In pairing tests of the 35 isolates for their vegetative comaptibility, 25 were heterokaryon self compatible and could be classified into 20 VCG S. Of them, 17 VCG S were represented by only a single isolate, whereas one VCG covered 4 isolates, and another two VCG S covered 2 isolates, respectively. The genetic diversity indexes for the members belonging to 'D', 'E' and 'F' mating populations were 0.7,0.8 and 1.0,respectively. It is concluded that isolates of Fusarium section Liseola from the diseased rice were genetically diverse.

关 键 词：水稻恶苗病 水稻恶苗病菌 遗传多样性 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]