Preliminary Analysis of Population Genetic Diversity of Cultivated Laminaria japonica Sporophyte via AFLP Technique  

作　　者：YI Heng SUI Zhenghong BAO Zhenmin 

机构地区：[1]Key Laboratory of Marine Genetics, Ministry of Education, Ocean University of China, Qingdao266003, P. R. China [2]Yunnan Henderson Foreign Language School, Kunming 650000, P. R. China

出　　处：《Journal of Ocean University of China》2010年第1期53-58,共6页中国海洋大学学报（英文版）

基　　金：funded by the ‘908’ Marine Survey Project of Shandong Province (SD-908-01-01-05.06)

摘　　要：The amplified fragment length polymorphic DNA (AFLP) technique was adopted to estimate the population genetic polymorphism among 30 sporophytes of Laminaria japonica collected from a cultivating farm in Rongcheng,China.Three methods were used for genomic DNA extraction from Laminaria japonica sporophyte and only the products obtained using the improved genomic DNA extraction kit method proved qualified for AFLP analysis.The parameters of the method were optimized.Samples of forty milligrams and the cell lysis time of 120 min were suggested to replace the parameters recommended by the manufacturer.Thirty individuals of Laminaria japonica from the same cultivating site were investigated using one pair of selective primers.A total of 21 loci were obtained and 17 of them were polymorphic.The mean percent age of polymorphic loci of this population was 80.95%.The Nei's gene diversity (H) within this population was 0.3028 and the average Shannon's Information index (I) was 0.4498.A genetic distance matrix among different individuals was constructed as well.Through this study,an applicable AFLP genetic analysis working system for Laminaria japonica sporophyte was established.The results of this research also revealed a high level of genetic diversity within the studied population.The amplified fragment length polymorphic DNA （AFLP） technique was adopted to estimate the population genetic polymorphism among 30 sporophytes of Laminariajaponica collected from a cultivating farm in Rongcheng, China. Three methods were used for genomic DNA extraction from Laminaria japonica sporophyte and only the products obtained using the improved genomic DNA extraction kit method proved qualified for AFLP analysis. The parameters of the method were optimized. Samples of forty milligrams and the cell lysis time of 120min were suggested to replace the parameters recommended by the manufacturer. Thirty individuals of Laminariajaponica from the same cultivating site were investigated using one pair of selective primers. A total of 21 loci were obtained and 17 of them were polymorphic. The mean percent age of polymorphic loci of this population was 80.95%. The Nei＇s gene diversity （H） within this population was 0.3028 and the average Shannon＇s Information index （I） was 0.4498. A genetic distance matrix among different individuals was constructed as well. Through this study, an applicable AFLP genetic analysis working system for Laminariajaponica sporophyte was established. The results of this research also revealed a high level of genetic diversity within the studied population.

关 键 词：SPOROPHYTE Laminariajaponica AFLP genetic diversity 

分 类 号：Q78[生物学—分子生物学] S82[农业科学—畜牧学]