A new strain Leptospirillum ferriphilum YTW315 for bioleaching of metal sulfides ores  

作　　者：张瑞永 夏金兰 彭娟花 张倩 张成桂 聂珍媛 邱冠周 

机构地区：[1]School of Minerals Processing and Bioengineering, Central South University [2]Key Laboratory of Biometallurgy of Ministry of Education, Central South University

出　　处：《中国有色金属学会会刊：英文版》2010年第1期135-141,共7页Transactions of Nonferrous Metals Society of China

基　　金：Projects(50621063, 50674101) supported by the National Natural Science Foundation of China;Project (2004CB619204) supported by the National Basic Research Program of China

摘　　要：A new strain named YTW315 was isolated from Dexing area using the double-layer culture technique. The morphological, biochemical and physiological characteristics of YTW315 were studied. Physiological investigation indicates that the strain YTW315 is a strict (obligate) chemolithoautotroph, metabolizing ferrous iron and pyrite. The optimal growth conditions for the strain are 40 ℃ and pH 1.6. A phylogenetic analysis based on 16S rRNA sequences shows that the isolate is clustered to Leptospirillum ferriphilum with 99.8% similarity to Leptospirillum ferriphilum strain Fairview and ATCC 49881. The molar fraction of DNA (G+C) of the isolate is 58.1%. The strain can tolerate high concentration of Fe(Ⅲ) and As(V) (500 mmol/L and 50 mmol/L, respectively). Bioleaching experiment indicates that the strain can oxidize Fe(Ⅱ) efficiently, and after 30 d, 44.56% of copper and 95.31% of iron are extracted from chalcopyrite and pyrite, respectively.A new strain named YTW315 was isolated from Dexing area using the double-layer culture technique. The morphological, biochemical and physiological characteristics of YTW315 were studied. Physiological investigation indicates that the strain YTW315 is a strict （obligate） ehemolithoantotroph, metabolizing ferrous iron and pyrite. The optimal growth conditions for the strain are 40 ~C and pH 1.6. A phylogenetic analysis based on 16S rRNA sequences shows that the isolate is clustered to Leptospirillumferriphilum with 99.8% similarity to Leptospirillumferriphilum strain Fairview and ATCC 49881. The molar fraction of DNA （G＋C） of the isolate is 58.1%. The strain can tolerate high concentration of Fe（III） and As（V）（500 mmol/L and 50 mmol/L, respectively）. Bioleaching experiment indicates that the strain can oxidize Fe（II ） efficiently, and after 30 d, 44.56% of copper and 95.31% of iron are extracted from chalcopyrite and pyrite, respectively.

关 键 词：生物浸出 硫化物矿石 病毒 RRNA基因序列 金属 最佳生长条件 系统发育分析 生理生化 

分 类 号：TD95[矿业工程—选矿]