他扎罗汀和NB-UVB对角质形成细胞他扎罗汀诱导基因1 mRNA的影响  

Effects of Tazarotene and Narrow-Band UVB on Tazarotene-induced Gene 1 mRNA Level in Normal Human Keratinocytes

作　　者：罗素菊[1] 刘全忠[1] 郑焱[2] 彭振辉[2] 倪海洋[1] 李燕[1] 

机构地区：[1]天津医科大学总医院皮肤科,天津300052 [2]西安交通大学医学院第二附属医院皮肤科,陕西西安710004

出　　处：《中国皮肤性病学杂志》2010年第1期5-7,60,共4页The Chinese Journal of Dermatovenereology

摘　　要：目的研究他扎罗汀和窄谱中波紫外线（NB—UVB）对培养的正常人角质形成细胞他扎罗汀诱导基因1（TIGI）mRNA表达的影响．方法用10^-7～10^-6mol／L他扎罗汀和／或50～100mJ／cm^2NB—UVB处理培养的正常人角质形成细胞24h后，用普通RT—PCR和实时荧光定量RT—PCR法检测角质形成细胞TIG1 mRNA水平的改变。结果正常人角质形成细胞可表达少量的TIG1 mRNA，10^-7mol／L和10^-6mol／L他扎罗汀单独处理角质形成细胞24h后，TIG1 mRNA的水平升高；50mJ／cm^2和100mJ／cm^2NB—UVB单独照射角质形成细胞24h后，TIG1 mRNA的水平无明显变化；二者联合作用时，TIG1 mRNA的水平明显升高，且高于二者单独处理时的水平（P〈0．01）。结论他扎罗汀和NB—UVB联合作用时可能通过协同上调TIG1的表达而发挥二者对角质形成细胞功能的调节作用、Objective To investigate the alteration of tazarotene-lnduced gene 1 （ TIG1 ） mRNA in cultured normal human keratinocytes after treatment with tazarotene and narrow-band UVB （NB-UVB） combined or alone. Methods Normal human keratinocytes were exposed to NB-UVB （50 - 100mj/cm^2） and/or followed incubation with tazarotene （10^-7- 10^-6mol/L） for 24 hours, then the level of TIG1 mRNA was examined by RT-PCR and real-time quantitative RT-PCR. Results TIG1 mRNA was enhanced by both doses of tazarotene but not by NB-UVB irradiation alone. Tazarotene-NB-UVB combination showed greater effects on increasing TIG1 mRNA, which were stronger than those induced separately（P 〈 0.01 ）. Conclusion Tazarotene-NB-UVB combination showed synergistic effects on increasing TIGI expression, through which they may play synergistic roles in accommodating keratinocytes function.

关 键 词：角质形成细胞 他扎罗汀 他扎罗汀诱导基因 中波紫外线 

分 类 号：R986[医药卫生—药品]