利用分子标记EPT11_(900)辅助甘蓝显性雄性不育基因转育  

Assisted Transgression of Dominant Male Sterile Gene (Ms) in Cabbage by Using Molecular Marker EPT11 900

作　　者：王晓武[1] 方智远[1] 孙培田[1] 刘玉梅[1] 杨丽梅[1] 庄木[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所

出　　处：《中国蔬菜》1998年第6期1-14,共14页China Vegetables

基　　金：国家自然科学基金

摘　　要：利用与甘蓝显性雄性不育基因（Ｍｓ）连锁的延长随机引物扩增ＤＮＡ（ＥＲＰＡＤ）标记ＥＰＴ１１９００对３３个甘蓝自交系的多态性进行了分析。ＥＰＴ１１９００主要分布在各种早熟甘蓝中，很少存在于鸡心类型或晚熟扁圆类型甘蓝中。该结果表明：它可用于辅助大多数鸡心型或晚熟扁圆类型甘蓝的Ｍｓ基因转育；ＥＰＴ１１９００在辅助两个甘蓝自交系回交三代及两个青花菜自交系回交一代的Ｍｓ基因转育中，预测的准确性超过９０％。Polymorphism was analyzed in 33 inbred lines of cabbage by using an extended random primer amplified DNA (ERPAD) marker EPT11 900 linked to a dominant male sterile gene in cabbage. The results indicated that EPT11 900 mainly distributed among inbred lines with early maturity and rarely existed in inbred lines with chicken-hearted head or late flat head. It was feasible to employ EPT11 900 in marker assisted transgression of Ms gene to most lines with late maturity or with chicken-hearted head. Predicted precision was more than 90% when EPT11 900 was used to assist transgression of Ms gene in BC3 generation of two cabbage lines and BC1 generation of one broccoli lines.

关 键 词：甘蓝 显性雄性不育 ERPAI 辅助选择 

分 类 号：S635.035[农业科学—蔬菜学]