扩展莫尼茨绦虫DAZ基因的分离和cDNA序列分析  

The Isolation and Sequence Analysis of the cDNA Encoding Moniezia expansa Deleted in Azoospermia

作　　者：赵文娟[1,2] 朱建军[1,2] 吕廷德[1,2] 薄新文[2] 王新华[2] 

机构地区：[1]石河子大学动物科技学院,石河子832000 [2]新疆兵团绵羊繁育生物技术重点实验室,石河子832000

出　　处：《石河子大学学报（自然科学版）》2010年第1期37-42,共6页Journal of Shihezi University(Natural Science)

基　　金：国家重点基础研究发展计划前期研究专项(2006CB708512)

摘　　要：为进一步研究扩展莫尼茨绦虫并以其作为模式生物提供基础。通过构建的扩展莫尼茨绦虫成虫cDNA文库,随机挑取重组阳性克隆进行测序,对部分序列进行引物步移法测序,获取其全长cDNA序列;采用生物信息学等技术对该cDNA序列进行开放阅读框(ORF)的寻找、编码氨基酸的推导、核苷酸和氨基酸同源性比较以及蛋白质结构的初步预测。结果显示:获得了1个扩展莫尼茨绦虫新基因DAZ基因,cDNA全长1905bp,包含1个完整的ORF,编码562个氨基酸,登录号为:GH291478。蛋白理论分子量为61.3169kD,等电点为4.77,不稳定系数50.27,脂肪系数65.04,总平均亲水性-0.472。二级结构预测该蛋白有1个跨膜区域,以无规卷曲(L)为主,没有二硫键结合位点。本研究成功分离扩展莫尼茨绦虫DAZ基因。To provide a foundation for further research even as model organisms, clones were selected randomly from the cDNA library and sequenced by using the method of expression sequence tags （ESTs）. Novel genes were acquired by primer-walking. By using hioinformaties, the cDNA sequence encoding M. expansa deleted in azoospermia（DAZ） was analyzed,including searching the ORF, translating the nucleotide to protein sequence,searching similarity and predicting of initial protein structure. The results showed that an new gene of M. expansa--DAZ gene obtained：the full-length of cDNA was 1905bp,a complete ORF encoding 562 amino acids,accession number was GH291478. The academic pI was 4.77 and molecular weight was 61. 3169kDa ,instability index. 50.27 ,aliphatic index. 65.04 ,grand average of hydropathicity： -0. 472. The conclusion is that：DAZ gene was successfully separated from M. expansa.

关 键 词：扩展莫尼茨绦虫 DAZ基因 序列分析 

分 类 号：S858[农业科学—临床兽医学]