山东宁阳大枣快繁体系的建立  

Establishment of Tissue Culture and Rapid Propagation System of Ningyang Chinese Date

作　　者：冯殿齐[1] 刘静[1] 赵进红[1] 张虹[1] 黄艳艳[1] 王玉山[1] 

机构地区：[1]山东省泰安市泰山林业科学研究院,山东泰安271000

出　　处：《中国园艺文摘》2010年第2期6-9,共4页Chinese Horticulture Abstracts

摘　　要：为探讨山东宁阳大枣组培快繁技术,以宁阳大枣新生枝条为外植体,通过诱导愈伤组织形成及不定芽分化,对建立高效的快繁体系进行研究。试验结果表明:同一基因型的不同取材部位其培养效果间差异不大;枣树新生枝条是建立无菌外植体的合适的外植体材料,适宜枣树分化愈伤组织的培养基是MS+BA1.0mg/L+KT1.0mg/L+NAA0.01mg/L+琼脂0.75%+蔗糖3%。适宜不定芽诱导的适宜培养基是MS+BA1.0mg/L+KT1.0mg/L+NAA0.05mg/L+琼脂0.75%+蔗糖3%。To study the establishment of high efficient regeneration system of Ningyang Chinese date, its new shoots were used as explants to induce callus and differentiate adventitious buds. The results showed that new shoots of jujube were suitable for explants.MS＋BA1.0mg/L＋KT1.0 mg/L＋NAA0.01 mg/L was suitable for inducing callus; MS＋BA1.0mg/ L＋KT1.0 mg/L＋NAA0.05 mg/L was suitable for cluster buds.

关 键 词：枣树 外植体 愈伤组织 再生植株 

分 类 号：S792.39[农业科学—林木遗传育种] S665.1[农业科学—林学]