牛生长激素抗独特型抗体促生长作用机理研究——兔抗牛生长激素抗体纯化与鉴定  

A Study on mechanism of Stimulating Growth ofAnti Idiotypic Antibodies Induced by RecombiantBovine Growth Hormone(rbGH):Purificiation and Characterization of Antibodies against Recombiant Bovine Growth Hormone(rbGH)

作　　者：高民[1] 刘晓松[1] 杨玉莹[1] 卢炳奎[1] 卢德勋[1] 

机构地区：[1]内蒙古畜牧科学院

出　　处：《中国兽医杂志》1998年第11期5-7,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金

摘　　要：选用８只新西兰白兔来制备牛生长激素抗独特型抗血清，初次免疫以完全弗氏佐剂乳化抗原，加强免疫用不完全弗氏佐剂乳化，最后一次加强注射后１０天采血，采用酶联免疫吸附试验来测定抗血清滴度和血清阻断率：３只兔血清抗体滴度分别为１１２８０００，１５１２０００和１１２８０００，而血清阻断率在血清稀释率为１２０００到１２５６０００时处于２７．７％到９２．９％范围。随后用硫酸铵沉淀结合ＤＥＡＥ纤维素层析对血清抗体进行了纯化，醋酸纤维素薄膜电泳试验结果表明ＩｇＧ成分获得了纯化。Eight rabbits were used to produce antiserum to recombiant bovine growth hormone(rbGH).The rabbits were immunized with rbGH initially emulsified in Freund' s complete and subsequenly imcomplete adjuvant,the antiserum was obtained at the 10th day after final booster injection.Indirect ELISA technique was used to monitor the antiserum titer and blocking rate. The results showed that the titer were 1∶128000,1∶512000 and 1∶128000 in the three rabbits,respectively,and the antiserum blocking rate were from 27.7 to 92.9% for the antiserum dilution from 1∶2000 to 1∶256000 Subsequently.IgG fraction in antiserum were purified by ammonium sulphate precipitation(35% saturation for final solution)and then subjected to a DEAE colume chromatography.Finally,purified Ig fractions were characterized by acetate cellulose membrane eletrophorese.The results indicated that the IgG fraction in antiserum have been purified.

关 键 词：牛生长激素 纯化 鉴定 作用机理 

分 类 号：Q575.110.3[生物学—生物化学]