乌司他丁对脓毒症大鼠肺微血管通透性及肺组织VEGF表达的影响  

Effect of Ulinastatin injection on the permeability of pulmonary microvascular and expression of vascular endothelial growth factor in lung tissue of sepsis in rats

作　　者：蔡施霞[1] 邓烈华[1] 佟琳[1] 孙小聪[1] 曾庆春[1] 

机构地区：[1]广东医学院附属医院危重医学科,524001

出　　处：《中国实用医药》2010年第2期1-3,共3页China Practical Medicine

基　　金：广东医学院青年基金项目(基金项目:2005307)

摘　　要：目的观察乌司他丁注射液对脓毒症大鼠肺微血管通透性及肺组织血管内皮生长因子(VEGF)表达的影响,探讨乌司他丁对脓毒症大鼠肺保护的作用机制。方法30只健康的雄性SD大鼠随机分为3组:假手术组(A组)、阳性对照组(B组)、乌司他丁治疗组(C组),每组10例。除假手术组外,其他组均施行盲肠结扎穿孔术(CLP)复制大鼠脓毒症模型,治疗组同时给予乌司他丁腹腔注射(100ku/kg),术后4h随机处死大鼠5只取组织标本,剩余5只大鼠经颈外静脉注射伊文思蓝观察肺微血管通透性。分别采用逆转录聚合酶链反应(RT-PCR)法和酶联免疫吸附(ELISA)法检测肺组织中VEGF的表达。结果B组肺微血管通透性及肺组织VEGF表达水平明显高于A、C组;C组肺微血管通透性及肺组织VEGF表达水平高于A组(P<0.05)。结论乌司他丁能明显抑制脓毒症大鼠肺微血管通透性增高,这种肺保护作用的机制可能与降低VEGF表达有关。Objective To observe the permeability of pulmonary microvascular and expression of vascular endothelial growth factor in septic rats treated with Ulinastatin injection, and to investigate the action mechanism of Ulinastatin in pulmonary function protect. Methods A total of 30 healthy male SD rats were randomly divided into three groups, and each group had 10 rats, Group A received sham operation and the others underwent cecal ligation and puncture （CLP） operation. All the rats were accepted intraperitoneal injection of normal saline （ group A, B） or Ulinastatin （100 ku/kg）. After four hours, Lung tissue express of VEGF in all rats were detected by reverse transcription-polymerase chain reaction（RT-PCR） and Enzyme-linked immunosorbent assay（ELISA） methods respectively and the permeability of pulmonary vascular were observed by injecting Evans Blue into jugular vein of rats. Results The permeability of pulmonary microvascular and the expression levels of VEGF in group B were significantly higher than those in group A and C ,which in group C were significantly higher than that in group A. Conclusions Ulinastatin is a reliable protection agent for septic induce acute lung injury, and its mechanism of action may be involved in the inhibition of VEGF expression.

关 键 词：乌司他丁注射液 脓毒症 肺微血管通透性 VEGF 

分 类 号：R459.7[医药卫生—急诊医学]