HPLC法同时测定三七花中人参皂苷Rb_1和三七皂苷Fe的含量  

HPLC simultaneous determination of ginsenoside Rb_1 and notoginsenoside Fe in flower buds of Panax notoginseng

作　　者：张冰[1] 陈晓辉[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2010年第2期233-235,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立HPLC法同时测定三七花中人参皂苷Rb1和三七皂苷Fe2种皂苷类成分的含量。方法:采用Promosil-C18(4.6mm×250mm,5μm)色谱柱,流动相为乙腈-0.05%磷酸水溶液,梯度洗脱,流速1.0mL.min-1,检测波长203nm,柱温30℃。结果:人参皂苷Rb1和三七皂苷Fe分别在51.21～307.3μg.mL-1(r=0.9995,n=6)和50.94～305.6μg.mL-1(r=0.9998,n=6)范围内呈良好线性关系;平均回收率(n=3)分别为97.9%～98.9%和97.0～99.4%。结论:本测定方法简便、快速、准确,为三七花质量评价提供了依据。Objective：To develop an RP-HPLC method for simultaneous determination of ginsenoside Rb1 and notoginsenoside Fe in flower buds of Panax notoginseng.Methods：The analysis was performed on a C18 column （4.6 mm×250 mm,5 μm） with gradient elution of acetonitrile-0.05% phosphoric acid solution at a flow rate 1.0 mL min-1.The UV detection wavelength was 203 nm,and the column temperature was set at 30 ℃.Results：The linear range was 51.21-307.3 μg/mL-1（r=0.9995,n=6） for ginsenoside Rb1 and 50.94-305.6 μg/mL-1（r=0.9998,n=6） for notoginsenoside Fe.The average recoveries（n=9） of ginsenoside Rb1 and notoginsenoside Fe were 97.9%-98.9% and 97.0-99.4% respectively.Conclusion：The method is found to be simple and accurate for simultaneous analysis of ginsenoside Rb1 and notoginsenoside Fe in flower buds of Panax notoginseng,and provided a reliable way for evaluating the quality of it.

关 键 词：三七花 人参皂苷RB1 三七皂苷Fe 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]