RP-HPLC法同时测定红花中羟基红花黄色素A与红花黄色素A的含量  

RP-HPLC determination of safflomin A and safflower yellow A in Carthamus tinctorius L

作　　者：姚苗苗[1] 任爱农[1,2] 董仲才 

机构地区：[1]江苏大学药学院,镇江212013 [2]江苏省中医药研究院,南京210028 [3]雅安三九药业有限公司,雅安625000

出　　处：《药物分析杂志》2010年第2期263-265,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立红花中羟基红花黄色素A与红花黄色素A的含量测定方法。方法:采用Akasil C18(4.6mm×250mm,5μm)色谱柱,以乙腈(A)-0.4%磷酸(B)为流动相,梯度洗脱(0～30min,10%A;30～50min,15%A;50～55min,25%A;55～60min,10%A),流速0.7mL.min-1,检测波长403nm,柱温30℃。结果:羟基红花黄色素A、红花黄色素A进样量的线性范围分别为0.451～1.05μg(r=0.9993)和0.278～0.650μg(r=0.9996)(n=5);平均加样回收率(n=6)分别为100.6%和103.0%。结论:本方法快速、灵敏,重复性好,适用于红花中羟基红花黄色素A与红花黄色素A的含量测定。Objective：To establish an RP-HPLC method for simultaneous determination of safflomin A and safflower yellow A in Carthamus tinctorius.Methods：The analytical column was Akasil C18 column（4.6 mm×150 mm,5 μm）.The mobile phase was composed of acetonitrile（A） and 0.4% phosphoric acid（B） with gradient elution（0-30 min,10%A 30-50 min,15%A 50-55 min,25%A 55-60 min,10%A） at a flow rate of 0.7 mL/min-1.The wavelength of UV detector was 403 nm and column temperature was 30 ℃.Results：The linear ranges of safflomin A and safflower yellow A were 0.451-1.05 μg（r=0.9993） and 0.278-0.650 μg（r=0.9996） （n=5）,respectively Their average recoveries（n=6） were 100.6% and 103.0%,respectively.Conclusion：This method is sensitive,repeatable and suitable to determine the contents of safflomin A and safflower yellow A in Carthamus tinctorius.

关 键 词：RP—HPLC 红花 羟基红花黄色素A 红花黄色素A 

分 类 号：R917[医药卫生—药物分析学]