HPLC-DAD法测定水母雪莲中牛蒡子苷元含量  

HPLC-DAD determination of arctigenin in Saussurea medusa Maxim

作　　者：于瑞涛[1,2] 罗智敏[1,2] 张兴旺[1,2] 陶燕铎[1] 梅丽娟[1] 邵赟[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810008 [2]中国科学院研究生院,北京100039

出　　处：《药物分析杂志》2010年第2期294-296,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技支撑计划项目(编号:2007BAI45B00)

摘　　要：目的:建立测定藏药水母雪莲中牛蒡子苷元含量的的HPLC法。方法:Eclipse XDB-C18色谱柱(4.6mm×150mm,5μm);流动相:乙腈-水(1:1),流速:1.0mL.min-1;检测波长:210nm;柱温:25℃。结果:牛蒡子苷元进样量在0.672～4.704μg范围内线性关系良好,其回归方程为:Y=4577.98837X+122.00,r=0.9999,牛蒡子苷元的含量为7.078mg.g-1。结论:本方法简便、快速,具有良好的精密性和重复性。Objective：To develop a reversed-phase HPLC method for the determination of arctigenin in Saussurea medusa Maxim.Methods：Eclipse XDB-C18 （150 mm×4.6 mm,5 μm）column was adopted with the mobile phase acetonitrile-water （1∶1） The flow rate was 1 mL/min-1,and the wavelength was set at 210 nm.Results：The linear range of arctigenin was 0.672-4.704 μg,the standard curve：Y=4577.98837X＋122.00,r=0.9999.The determination of arctigenin in Saussurea medusa Maxim.was 7.078 mg/g-1.Conclusions：The method is simple,fast and accurate with good reproducibility.

关 键 词：水母雪莲 牛蒡子苷元 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]