HPLC法测定愈伤灵胶囊中羟基红花黄色素A的含量  

HPLC determination of hydroxysafflor yellow A in Yushangling capsules

作　　者：陈宗良 朱克 周玲娜 豆久锋[2] 吴锋[2] 

机构地区：[1]浙江省金华市食品药品检验所,金华321000 [2]浙江大学医学院附属第一医院,杭州310003

出　　处：《药物分析杂志》2010年第2期297-299,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱法测定愈伤灵胶囊中羟基红花黄色素A含量。方法:采用Zorbax Eclipse XDB-C18(150mm×4.6mm,5μm)色谱柱,流动相为甲醇-0.5%磷酸溶液(25:75),流速1.0mL.min-1,检测波长403nm,柱温35℃。结果:羟基红花黄色素A进样量在0.105～2.10μg范围内呈良好的线性关系(r=0.9999);平均回收率(n=6)为98.6%。结论:本法操作简便,专属性强,灵敏度高,重复性好,可用于愈伤灵胶囊中羟基红花黄色素A的含量控制。Objective：To develop an HPLC method for determination of hydroxysafflor yellow A in Yushangling capsules.Methods：The Zorbax Eclipse XDB-C18（150 mm×4.6 mm,5 μm） column was adopted.The mobile phase consisted of methanol-0.5% phosphoric acid（25∶75） at a flow rate of 1.0 mL/min-1.The detection wavelength was 403 nm and the column temperature was 35 ℃.Results：The calibration curve was in good linearity within the range of 0.105 to 2.10 μg（r=0.9999）.The average recovery（n=6） of hydroxysafflor yellow A was 98.6%.Conclusion：The method is simple and accurate.It can be used for content control of hydroxysafflor yellow A in Yushangling capsules.

关 键 词：高效液相色谱法 愈伤灵胶囊 羟基红花黄色素A 含量测定 

分 类 号：R917[医药卫生—药物分析学]