RP-HPLC法同时测定银莲提取物中5种指标成分的含量  

RP-HPLC determination of 5 index components in Yinlian extract

作　　者：刘洁琼[1] 俞嘉[1] 熊志立[1] 李发美[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2010年第1期12-16,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家中医药管理局中医药科学技术研究专项项目(04-05ZP14)

摘　　要：目的:建立反相高效液相色谱法同时测定银莲提取物中绿原酸、荭草苷、牡荆苷、蒙花苷和木犀草素的含量。方法:采用Thermo Hypersil BDS C18(250 mm×4.6 mm,5μm)色谱柱,流动相为0.4%醋酸水溶液(A)-乙腈(B),梯度洗脱(0～25min,93%A→70%A;25～35 min,70%A),流速为0.7 mL.min-1,检测波长为330 nm,柱温为室温。结果:银莲提取物中绿原酸、荭草苷、牡荆苷、蒙花苷和木犀草素浓度分别在0.290～11.6μg.mL-1(r=0.9998)、0.350～14.0μg.mL-1(r=0.9997)、0.320～12.8μg.mL-1(r=0.9997)、0.280～11.2μg.mL-1(r=0.9995)、0.110～4.40μg.mL-1(r=0.9999)的范围内与峰面积呈良好线性关系;方法的回收率(n=6)分别为99.4%,106%,102%,106%,92.3%,其RSD分别为2.6%,2.1%,2.8%,1.4%,1.5%。结论:本法快速、简便、准确,重复性好,适用于银莲提取物的质量控制。Objective：To develop an RP-HPLC method for simultaneous determining chlorogenic acid,orientin,vitexin,linarin and luteolin in Yinlian extract.Methods：The separation was carried out on Thermo Hypersil BDS C18（250 mm×4.6 mm,5 μm） column with a mobile phase of 0.4% acetic acid solution（A） and acetonitrile（B） in gradient mode（The initial ratio of 0.4% acetic acid solution was 93% and decreased to 70% within 25 minutes,and the ratio of 0.4% acetic acid solution was maintained 70% within following 10 minutes）,at a flow rate of 0.7 mL·min-1.The detection wavelength was set at 330 nm and the analysis was performed at room temperature.Results：The linear ranges of chlorogenic acid,orientin,vitexin,linarin and luteolin were 0.290-11.6 μg·mL-1（r=0.9998）,0.350-14.0 μg·mL-1（r=0.9997）,0.320-12.8 μg·mL-1（r=0.9997）,0280-11.2 μg·mL-1（r=0.9995）,0.110-4.40 μg·mL-1（r=0.9999）,respectively.The average recoveries（n=6）of chlorogenic acid,orientin,vitexin,linarin and luteolin was 99.4%,106%,102%,106%,92.3% and with RSD of 2.6%,2.1%,2.8%,1.4%,1.5%,respectively.Conclusion：This method is fast,simple,reproducible for the simultaneous determination of chlorogenic acid,orientin,vitexin,linarin and luteolin in Yinlian extract.

关 键 词：反相高效液相色谱 银莲提取物 绿原酸 荭草苷 牡荆苷 蒙花苷 木犀草素 

分 类 号：R917[医药卫生—药物分析学]