RP-HPLC法测定妇科栓剂中氧化苦参碱、苦参碱和小檗碱含量  

RP-HPLC determination of oxymatrine,matrine and berberine in Fuke suppository

作　　者：黄松[1,2] 陈吉航[1] 陶艳[1] 赵薇[1] 罗明琍[1] 赖小平[1,2] 

机构地区：[1]广州中医药大学新药开发研究中心,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,东莞523808

出　　处：《药物分析杂志》2010年第1期110-113,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱法同时测定妇科栓剂中氧化苦参碱、苦参碱和小檗碱3个有效成分的含量。方法:采用Kro-masil C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-乙腈-乙酸铵缓冲溶液(浓氨水调pH至8.5)为流动相进行梯度洗脱,流速1.0 mL.min-1,检测波长220 nm(氧化苦参碱、苦参碱)、265 nm(小檗碱),柱温为室温。结果:氧化苦参碱、苦参碱和小檗碱线性范围分别为0.095～2.37μg(r=0.9999),0.129～3.22μg(r=0.9999),0.362～9.05μg(r=0.9999);平均加样回收率(n=5)分别为100.2%,99.2%,98.9%。结论:本方法的精密度、稳定性、重复性和加样回收率均较好;所建立的HPLC方法准确、快速,可用于妇科栓剂中氧化苦参碱、苦参碱和小檗碱3个主要成分的含量测定。Objective：To develop an HPLC method for determination of three components（oxymatrine,matrine and berberine） in Fuke suppository.Methods：Analysis was performed on a Kromasil C18 column（250 mm×4.6 mm,5 μm） with the mixture of methanol,acetonitrile and ammonium acetate buffer solution（adjust pH to 8.5 with stronger ammonia water） as mobile phase in gradient elution,and the flow rate was 1.0 mL·min-1;The detection wavelength was 220 nm for oxymatrine and matrine and 265 nm for berberine;The column temperature was room temperature.Results：The calibration curves of oxymatrine,matrine and berberine were linear in the ranges of 0.095-2.37 μg（r=0.9999）,0.129-3.22 μg（r=0.9999）,0.362-9.05 μg（r=0.9999）,respectively;The average recoveries（n=5） of the three components in Fuke suppository were 100.2%,99.2%,98.9%,respectively.Conclusion：The accuracy,stability,repeatability,and average recovery of the method are satisfying,and the three components（oxymatrine,matrine and berberine） of Fuke suppository can be rapidly and accurately quantified by HPLC.

关 键 词：妇科栓剂 氧化苦参碱 苦参碱 小檗碱 RP—HPLC 

分 类 号：R917[医药卫生—药物分析学]