微波技术在乳癖消片液相色谱分析中的应用  

Application of microwave technique to analysis with HPLC to Rupixiao tablets

作　　者：李洪书 邓春晖[2] 陈斌 

机构地区：[1]上海市闸北食品药品检验所,上海200435 [2]复旦大学,上海200433

出　　处：《药物分析杂志》2010年第1期138-141,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:应用微波技术处理后以高效液相色谱法测定乳癖消片中三七皂苷R1和人参皂苷Rg1的含量。方法:以水为溶剂,以微波回流的方式提取乳癖消片中三七皂苷R1和人参皂苷Rg1,制成供试品溶液,以Agilent Eclipse XDB-C18(4.6 mm×150 mm,5μm)为色谱柱,乙腈-0.05%磷酸溶液(20∶80)为流动相,流速0.72 mL.min-1,柱温20℃,检测波长203nm,参比波长360 nm,并在400～190 nm做全波长扫描。结果:在本色谱条件下,三七皂苷R1和人参皂苷Rg1可得到完全分离,三七皂苷R1的浓度在49.25～492.5μg.mL-1、人参皂苷Rg1的浓度在53.00～530.0μg.mL-1范围内与峰面积均呈良好的线性关系,相关系数均为0.9999;回收率(n=6)分别为98.19%和101.5%。结论:本方法简便、准确,重复性较好,其主要特点是快速、节能、节省溶剂、污染小,从而降低了劳动强度,改善了工作环境。Objective：To determine the content of notoginsenoside R1 and ginsenoside Rg1 in Rupixiao tablets by HPLC after applying microwave technique to deal with the sample.Methods：The distilled water as the solvent,to extract notoginsenoside R1 and ginsenoside Rg1 in Rupixiao tablets with the microwave circumfluence,and receiving the sample solution,an Agilent Eclipse XDB-C18 column（4.6 mm×150 mm,5 μm）was adopted with a mixture of acetonitrile and 0.05% phosphoric acid solution（20∶80）as the mobile phase at a flow rate of 0.72 mL·min-1 and the column temperature of 20 ℃ with the detection wavelength at 203 nm,the reference wavelength of 360 nm,and the whole wavelength scanning between 400 nm and 190 nm.Results：With this chromatographic condition,notoginsenoside R1 and ginsenoside Rg1 can be separated completely.The calibration curve was linear in the range of 49.25-492.5 μg·mL-1 for notoginsenoside R1 with r=0.9999 and 53.00-530.0 μg·mL-1 for ginsenoside Rg1 with r=0.9999;The average recoveries（n=6）of notoginsenoside R1 and ginsenoside Rg1 were 98.19% and 101.5%,respectively.Conclusion：The method is simple,accurate,and reproducible,the primary characteristic is fast,saving on the energy and the solvent,and the contamination is fewer,so it can reduce the labor intensity and improve the work circumstance. Key words：notoginsenoside R1;ginsenoside Rg1;Rupixiao tablets;microwave technology;HPLC

关 键 词：三七皂苷R1 人参皂苷RG1 乳癖消片 微波技术 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]