高氧预适应增强大鼠心肌Mn-SOD基因表达及缺血梗塞心肌SOD活力  

EFFECTS OF HYPEROXIC PRECONDITIONING ON THE EXPRESSION OF MnSOD GENE AND THE SOD ACTIVITYIN ISCHEMIC AND INFARCT MYOCARDIUM OF RATS

作　　者：李小鹰[1,2] 英明中[1,2] 赵亚力 田亚平[1,2] 谷志远 徐光华[1,2] 

机构地区：[1]解放军总医院 [2]海军总医院

出　　处：《中国应用生理学杂志》1998年第3期245-249,共5页Chinese Journal of Applied Physiology

摘　　要：本文报告了一种新的心脏预适应途径即高氧预适应（ｈｙｐｅｒｏｘｉｃｐｒｅｃｏｎｄｉｔｉｏｎｉｎｇ，ＨＯＰ）。为观察高氧预适应对鼠心肌抗氧化酶的影响，我们给大鼠吸入８０％～８５％氧气，１ａｔｍ，每天６ｈ，连续７ｄ（ＨＯ组），然后进行实验并与室内空气环境下喂饲７ｄ的大鼠（对照组）进行比较。３０只Ｗｉｓｔｅｒ大鼠随机分为６组，每组５只：ＨＯ－１和对照－１组，ＨＯ吸入结束后直接处死，采用Ｎｏｒｔｈｅｎ杂交法测定心肌Ｍｎ－ＳＯＤｍＲＮＡ表达；ＨＯ－２和对照－２，采用Ｌａｎｇｅｎｄｏｒｆ法造成６０ｍｉｎ缺血，６０ｍｉｎ再灌注，测心肌ＳＯＤ与冠脉回流液的ＣＰＫ及ＳＯＤ活力；ＨＯ－３和对照－３，同法造成１０ｍｉｎ缺血，６０ｍｉｎ再灌注，测心肌与冠脉回流液的ＳＯＤ活力。结果显示，ＨＯ－１组心肌Ｍｎ－ＳＯＤｍＲＮＡ表达明显较对照组增强。缺血及梗塞心肌ＳＯＤ的活力分别为（％／ｇ）：ＨＯ－２８３．６±０．５；对照－２７６．７±１．７（Ｐ＜０．０１），ＨＯ－３９０．１±１．９；对照－３８５．１±１．４（Ｐ＜０．０５）。ＨＯ组冠脉回流液的ＳＯＤ活力在缺血再灌注期明显高于对照组（Ｐ＜０．００１）。冠脉回流液ＣＰＫ活力ＨＯ－２组再灌注３０及?e report a new method of myocardial preconditioning named hyperoxic preconditionning(HOP). In order to study the effects of HOP on myocardial ischemic injury and antioxidant enzymes, the adult rats were preexposed to 80%～85% of oxygen, 1 atm, six hours per day for seven days before the experiments (HO groups). The control groups were in room air for seven days. Thirty Wister rats were devided randomly into six groups (n=5 for each group): the rats were killed afier the HO protocol and the expressions of MnSOD mRNA in myocardium were determined by Nonhern Blot (HO1 and controll); the isolated rat hearts with Langendorff method underwent 60min ischemia followed by 60min reperfusion (HO2 and control2) or 10min ischemia followed by 60min reperfusion(HO3 and control3). The activities of SOD in myocardium and in coronary efluent were measured in the last four groups,the activities of CPK in coronary efluent were measured in HO2 and control2 groups. The results showed that the expressions of MnSOD mRNA in myocardium were stronger in HOl group than that in controll group; the activities of SOD were significantly higher in HO2 and HO3 groups than those in control groups. The activities of CPK in coronary effluents were significantly lower in HO2 than those in control2 group at 30 and 60 min after reperfusion (P<0.05). Conclusion: hyperoxic preconditioning(HOP) can induce the increased expression of MnSOD mRNA and the activities of SOD in myocardium in rat.

关 键 词：高氧预适应 大鼠 心肌 MN-SOD 心肌病 

分 类 号：R459.6[医药卫生—治疗学] R542.205[医药卫生—临床医学]